CHARACTERIZATION OF THE COMPLETE CHLOROPLAST GENOME OF RORIPPA DUBIA (PERSOON) H. HARA (BRASSICACEAE）

Abstract

We employed high-throughput sequencing to decode the entire chloroplast (cp) genome of the medicinal annual herb Rorippa dubia. The genome is 154,735 bp in total length and exhibits a typical quadripartite structure, including a large single-copy (LSC) region of 83,736 bp, a small single-copy (SSC) region of 18,005 bp, and a pair of inverted repeats (IRs) of 26,497 bp. The overall guanine-cytosine (GC) content is 34.60%. The genome contains 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 27 complete chloroplast (cp) genomes from Brassicaceae, using the maximum likelihood (ML) method, showed that R. dubia formed a monophyletic group with other Rorippa species, strongly supporting its taxonomic position within the genus. As the inaugural report of a complete plastome for R. dubia, our findings establish an essential genomic framework that will facilitate subsequent investigations into the phylogeny and population genetics of the Rorippa genus.