STUDIES ON APOPTOSIS INDUCTION AND P53 GENE EXPRESSION AT IC50 CONCENTRATION OF A PETROLEUM ETHER EXTRACT OF A POLY HERBAL FORMULATION ON A549 CELL LINES

Abstract

A polyherbal formulation comprising extracts of seeds of Nigella sativa and fruits of Vitis vinifera, Cucumis sativus, Phoenix dactylifera, Punica granatum, and Ficus carica, using petroleum ether solvent. Using the MTT assay, this formulation's cytotoxic ability against A549 lung cancer cells was assessed, yielding an IC50 value of 87.25 µg/ml.

Remarkably, the petroleum ether extract's IC50 concentration caused A549 cells to undergo apoptosis, indicating a potent anti-cancer action. Additionally, the formulation increased the expression of the P53 gene, a crucial tumour suppressor gene that controls apoptosis and cell cycle progression.

Pursuant to the study's findings, the polyherbal concoction may have chemo-preventive attributes, and regular use of it as part of a regular diet or as a dietary supplement may help prevent cancer. The formulation may also be a useful source for the creation of innovative cancer treatments, which calls for more research and validation through clinical trials and in vivo investigations.