MOLECULAR PROFILING OF SIGNAL TRANSDUCTION ALTERATIONS IN DISEASE STATES

Abstract

Signal transduction pathways are essential in controlling cellular functions including proliferation, survival, differentiation and stress response. These pathways are dysregulated in a characteristic of many disease conditions, such as cancer, metabolic disorders, and inflammatory conditions. The molecular changes within signaling networks are important to understand to determine key disease progression drivers and potential therapeutic targets. The current research is expected to systematically profile signal transduction changes and elicit important regulatory elements in disease-linked molecular malfunction.

It used a detailed analytical method, which combined the use of gene expression profiling and protein-level signaling analysis. The differentially expressed genes were detected by RNA sequencing (RNA-seq) and RT-qPCR, and the activation status of the main signaling proteins was measured by the Western blot analysis. Analysis of functional enrichment with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) data sets was performed with the aim of identifying signaling pathways significantly changed.

The outcomes showed there was a notable dysregulation of key signaling pathways, such as PI3K-Akt, MAPK, NF-kB, and p53. Third, a number of signaling molecules were differentially expressed showing an upregulation of survival and proliferation-associated and downregulation regulatory and apoptotic signaling. Analysis of the network revealed that these pathways are extensively networked and also play a role in the development of diseases.

Conclusively, the paper indicates that the role of disease pathogenesis is played by disruption of signaling networks and not solitary pathway changes. The discovery of important regulatory pathways and hub signaling molecules offers meaningful information into the molecular pathways of disease states and has arbitered where therapeutic intervention could be directed.