RAPD AND ISSR MARKERS: MOLECULAR APPROACH TO SELECT DIVERSE PARENTS IN SUNFLOWER (HELIANTHUS ANNUUS L.) FOR NON-TRADITIONAL AREAS OF INDIA

Abstract

Considering the significance of sunflower cultivation under various agro-climatic conditions, an examination was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders. The estimation of similarity coefficient went from 0.41 to 0.87 for example 41-87% with genetic diversity went from 13-59%. The normal closeness across all genotypes was discovered to be 0.64, demonstrating that the genotypes were tolerably comparable genetically. The most extreme closeness estimation of 0.87 was seen between genotypes 234B and 2-7-1B whereas, same trend was also observed between CMS 234B and 89-1B. While EC 623023 and 6D 1R were discovered to be hereditarily assorted with least similitude estimation of 0.41. Genotype EC 623023 demonstrated low likeness esteems with the vast majority of the genotypes and subsequently was generally assorted from rest of the genotypes. RAPD and ISSR group tree examination of 14 sunflower genotypes indicated that they were predominantly separated into two fundamental clusters with a similitude coefficient of 0.61. The genotype EC 623023 was prohibited from any remaining genotypes. In view of RAPD and ISSR investigates, the genotypes EC 623023, R 16, (tester) and CMS 234B (line) were discovered to be genetically diverse. Most cross breeds of these parental lines showed huge SCA impacts, heterosis and high in per se performance.