BIOACTIVE CHARACTERIZATION OF JUGLANS REGIA L. BARK: A COMBINED UV-VIS AND FTIR SPECTROSCOPIC APPROACH

Abstract

Background: English walnut The bark of many plants is phenolic-rich botanical material that has ethnomedicinal significance and is gaining interest as a source of natural antioxidants and antimicrobial phytochemicals. The current study gives a qualitative spectroscopic and phytochemical analysis of J. The uploaded UV-Vis and FTIR spectral files provide the primary data source for the regia bark extract. The objective was to interpret the UV-Vis and FTIR fingerprints using probable bioactive constituents without making use of any quantitative or chromatographic data. The UV-Vis interpretation file, as discussed before, had an absorption band centred at 420 nm. The FTIR data had 3295.6, 2921.5, 1618.0, 1317.1, 1018.0 and 778.7 cm^-1 diagnostic bands. Standard principles of spectroscopic interpretation were used to assign the peaks which were then compared with published phytochemical data on phytoconstituents isolated from Juglans regia and plant phenolic. Results: The 420 nm UV-Vis band aligning with electronic transitions in conjugated chromophoric systems was interpreted as supportive evidence for phenolic and related aromatic constituents that included possible presence of ferulic acid, vanillic acid, coumaric acid, syringic acid and other conjugated metabolites. The bands observed in FTIR are due to the vibrations of bonds such as hydroxyl, aliphatic C-H, aromatic C=C/conjugated carbonyl, phenolic C-O, glycosidic/polysaccharide C-O-C, substituted aromatic ring vibrations (Cyclic) etc. Fingerprinting through combined UV-Vis and FTIR. There exist significant phenolic, flavonoid/tannin like, aromatic and glycosidic constituents in J. Extract of regia bark. Because compound identity requires confirmatory HPLC, LC-MS/MS, GC-MS analyses and, where appropriate, quantitative phytochemical assays these findings are preliminary.