Microsporogenesis in Brachiaria dictyoneura (Fig. & De Not.) Stapf (Poaceae: Paniceae)

C. Risso-Pascotto, M.S. Pagliarini, C.B. Valle
Published November 12, 2006
Genet. Mol. Res. 5 (4): 837-845 (2006)

About the authors
C. Risso-Pascotto, M.S. Pagliarini, C.B. Valle

Corresponding author
M.S. Pagliarini
E-mail: mspagliarini@uem.br


Microsporogenesis was analyzed in five accessions of Brachiaria dictyoneura presenting x = 6 as the basic chromosome number. All accessions were tetraploid (2n = 4x = 24) with chromosome pairing in bi-, tri-, and quadrivalents. The recorded meiotic abnormalities were those typical of polyploids, including precocious chromosome migration to the poles, laggard chromosomes, and micronucleus formation. The frequency of these abnormalities, however, was lower than those reported for other polyploid accessions previously analyzed for other Brachiaria species. Cell fusion and absence of cytokinesis were also recorded in some accessions, leading to restitutional nucleus formation in some cells. Genetically unbalanced microspores, binucleate, and 2n microspores were found among normal meiotic products as results from these abnormalities. The limitation in using these accessions as pollen donor in interspecific crosses with sexual species with x = 7 or x = 9 in breeding programs is discussed.

Key words: Brachiaria dictyoneura, Interspecific hybridization, Basic chromosome number, Microsporogenesis, Cell fusion, Cytokinesis.

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