Molecular epidemiology

Osteoprotegerin polymorphisms in Chinese Han patients with rheumatoid arthritis

X. H. Ye, Cheng, J. L., and Liu, R. P., Osteoprotegerin polymorphisms in Chinese Han patients with rheumatoid arthritis, vol. 14, pp. 6569-6577, 2015.

In order to investigate the association between osteoprotegerin (OPG) gene polymorphisms and rheumatoid arthritis (RA), we studied OPG rs3102735 T/C and rs2073618 G/C polymorphisms in a Chinese Han population comprising 574 patients with RA and 804 controls. Genotyping by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was conducted. Our data indicated that OPG rs3102735 T/C and rs2073618 G/C polymorphisms were not associated with the risk of RA.

Association among XRCC1, XRCC3, and BLHX gene polymorphisms and chromosome instability in lymphocytes from patients with endometriosis and ovarian cancer

M. S. Monteiro, Boas, D. B. Vilas, Gigliotti, C. B., and Salvadori, D. M. F., Association among XRCC1, XRCC3, and BLHX gene polymorphisms and chromosome instability in lymphocytes from patients with endometriosis and ovarian cancer, vol. 13, pp. 636-648, 2014.

Endometriosis is a complex disease that has both benign and malignant characteristics. It affects 5-10% of women of reproductive age. Studies have demonstrated the existence of common genetic changes in endometriosis and ovarian cancer, suggesting a possible association between these 2 diseases. However, the mechanisms that lead to the development of cancer from endometriosis remain unknown. In this study, we evaluated 3 groups of women: 72 patients with endometriosis, 70 with ovarian cancer, and 70 healthy individuals (controls).

Insulin-like growth factor binding protein-3 (IGFBP-3) genetic variant and the risk of esophageal squamous cell carcinoma in a Chinese population

H. - P. Yang, Liu, J. - F., Rao, J., Zhang, X. - M., Qian, H. - L., Niu, X. - Q., and Zhao, Z. - L., Insulin-like growth factor binding protein-3 (IGFBP-3) genetic variant and the risk of esophageal squamous cell carcinoma in a Chinese population, vol. 13, pp. 4146-4153, 2014.

Insulin-like growth factor binding protein-3 (IGFBP-3) exerts anti-proliferative or pro-apoptotic effects through IGF-dependent as well as IGF-independent mechanisms in vitro. The purpose of this study was to examine the association between genetic variants in IGFBP-3 (rs2270628) and the risk of esophageal squamous cell carcinoma (ESCC) in a Chinese Han population. Five hundred ESCC cases and 500 cancer-free controls of the Chinese Han population were involved in this study.

Genetic diversity of Yersinia pestis in Brazil

M. B. M. Oliveira, Barros, M. P. S., Silveira-Filho, V. M., Araújo-Nepomuceno, M. R., Balbino, V. Q., Leal, N. C., Almeida, A. M. P., and Leal-Balbino, T. C., Genetic diversity of Yersinia pestis in Brazil, vol. 11, pp. 3414-3424, 2012.

Plague outbreaks are occasionally reported in Brazil. Unfortunately, due to great genetic similarity, molecular subtyping of Yersinia pestis strains is difficult. Analysis of multiple-locus variable number of tandem repeats (VNTR), also known as MLVA, has been found to be a valuable tool to discriminate among strains.

Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis

M. P. S. Barros, Silveira-Filho, V. M., Lins, R. H. F. B., Oliveira, M. B. M., Almeida, A. M. P., and Leal-Balbino, T. C., Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis, vol. 12, pp. 1294-1302, 2013.

We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak strains, as identical pulsotype patterns were found among outbreak strains and strains obtained from surveillance. However, it was able to detect intraspecific genetic diversity among Brazilian strains.

Molecular epidemiology of the hepatitis C virus in Brazil

S. Busek and Oliveira, G., Molecular epidemiology of the hepatitis C virus in Brazil, vol. 2, pp. 117-123, 2003.

Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The genome of this virus consists of approximately 10,000 bp and codes for 10 mature polypeptides. Genome sequence comparison has revealed the existence of six major genotypes and a large number of subtypes. The genotypes can be distinguished by whole genome or genome fragment sequencing, geno-type specific amplification of a genomic region or PCR amplification, followed by hybridization or restriction digestion, among other methods.

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