Escherichia coli

Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories

C. B. Dallagassa, Huergo, L. F., Stets, M. I., Pedrosa, F. O., Souza, E. M., Cruz, L. M., Assis, F. E. A., Wolf, S., Volanski, W., Picheth, G., Pigatto-Denardi, C. P., Farah, S. M. S. S., and Fadel-Picheth, C. M. T., Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories, vol. 13, pp. 716-722, 2014.

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains.

Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli

A. Gholizadeh, Comparative fusion expression of maize SINAT5 in two different strains of Escherichia coli, vol. 11, pp. 2760-2768, 2012.

SINAT5 is a plant E3 ligase that regulates auxin signaling and root morphogenesis by ubiquitination of the NAC1 protein. Consequently, it may be a putative regulator of aspects of plant development cycles that are controlled by auxin. Efficient production, purification and correctly folded form of this protein are important requirements for functional studies. We produced and quantitatively compared fusion expression of the “maltose binding protein (mbp)-maize sinat5” construct in two different strains of Escherichia coli.

Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance

S. Ghanem, Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance, vol. 10, pp. 1445-1454, 2011.

In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E. coli DH5a ΔrecA (recombinant, deficient strain). Transferred cells were tested for ampicillin, tetracycline, kanamycin, neomycin, geneticin, paromomycin, penicillin, and UV resistance.

Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains

F. A. Oliveira, Paludo, K. S., Arend, L. N. V. S., Farah, S. M. S. S., Pedrosa, F. O., Souza, E. M., Surek, M., Picheth, G., and Fadel-Picheth, C. M. T., Virulence characteristics and antimicrobial susceptibility of uropathogenic Escherichia coli strains, vol. 10, pp. 4114-4125, 2011.

Eight virulence factors associated with uropathogenic Escherichia coli (UPEC) were investigated in 204 clinical isolates of E. coli recovered from urine cultures at counts ≥105. The bacteria were classified into two groups according to the number of leukocytes in urine samples from which they were isolated: group I ≤8 leukocytes/hpf, 104 strains; group II >8 leukocytes/hpf, 100 strains.

A genomic-scale search for regulatory binding sites in the integration host factor regulon of Escherichia coli K12

Tdos M. Santos and Rodrigues, P. Sérgio, A genomic-scale search for regulatory binding sites in the integration host factor regulon of Escherichia coli K12, vol. 4, pp. 783-789, 2005.

We examined general aspects of the DNA-protein interaction between the integration host factor (IHF) global regulator and its regulatory binding sites in the Escherichia coli K12 genome. Two models were developed with distinct weight matrices for the regulatory binding sites recognized by IHF. Using these matrices we performed a genome scale scan and built a set of computationally predicted binding sites for each of the models. The sites found by the model associated with repetitive sequences had a higher score in the sequence to matrix alignment.

DNA sequencing of a pathogenicity-related plasmid of an avian septicemic Escherichia coli strain

E. G. Stehling, Campos, T. A., Azevedo, V., Brocchi, M., and Silveira, W. D., DNA sequencing of a pathogenicity-related plasmid of an avian septicemic Escherichia coli strain, vol. 6, pp. 331-337, 2007.

A 43-MDa conjugative plasmid isolated from an avian septicemic Escherichia coli (APEC) strain possessing genes related to the adhesion and invasion capacities of in vitro-cultured cells was sequenced. The results demonstrated that the 43-MDa plasmid harbors bacterial pathogenicity-related sequences which probably allow the wild-type pathogenic strain to adhere to and invade tissues and to cause septicemia in poultry.

Genome-wide partial correlation analysis of Escherichia coli microarray data

D. F. T. Veiga, Vicente, F. F. R., Grivet, M., de la Fuente, A., and Vasconcelos, A. T. R., Genome-wide partial correlation analysis of Escherichia coli microarray data, vol. 6, pp. 730-742, 2007.

Transcriptional control is an essential regulatory mechanism employed by bacteria. Much about transcriptional regulation remains to be discovered, even for the most widely studied bacterium, Escherichia coli. In the present study, we made a genome-wide low-order partial correlation analysis of E. coli microarray data with the purpose of recovering regulatory interactions from transcriptome data.

Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms

L. Augusto-Pinto, da Silva, C. Gustavo Re, Lopes, Dde Oliveir, Machado-Silva, A., and Machado, C. Renato, Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms, vol. 2. pp. 77-91, 2003.

The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examined tools used for gene cloning and functional studies of DNA repair in eukaryotes, using this bacterial system as a model.

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