Cross-amplification

Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus

G. Chen, Huang, S., Gao, J., Bai, X., Wang, W., and Cao, X., “Development and characterization of microsatellite markers via cross-species amplification of Paramisgurnus dabryanus”, vol. 14, pp. 5694-5698, 2015.

The large-scale loach, Paramisgurnus dabryanus, is a small freshwater fish of major economic importance in many Asian countries, particularly China and South Korea. Fifteen polymorphic microsatellite (simple sequence repeat) markers were obtained through cross-species amplification between this loach and a related species, Misgurnus anguillicaudatus (GenBank accession numbers: KC117456 to KC117470). The number of alleles per locus ranged from 5 to 12 among 40 individuals, and the average observed and expected heterozygosities were 0.344 and 0.828, respectively.

New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis

C. X. Tian, Liang, X. - F., Yang, M., Dou, Y. Q., Zheng, H. Z., Cao, L., Yuan, Y. C., and Zhao, C., “New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis”, vol. 13, pp. 546-558, 2014.

The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The polymorphic information content value varied from 0.255 to 0.782. Twelve microsatellite loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni’s correction.

Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction

E. M. Martins, Martinelli, G., Arbetman, M. P., Lamont, R. W., Simões-Araújo, J. L., Powell, D., Ciampi-Guillardi, M., Baldauf, C., Quinet, A., Galisa, P., and Shapcott, A., “Development and characterization of microsatellite loci for Ocotea species (Lauraceae) threatened with extinction”, vol. 13, pp. 5138-5142, 2014.

The Atlantic rainforest species Ocotea catharinensis, Ocotea odorifera, and Ocotea porosa have been extensively harvested in the past for timber and oil extraction and are currently listed as threatened due to overexploitation. To investigate the genetic diversity and population structure of these species, we developed 8 polymorphic microsatellite markers for O. odorifera from an enriched microsatellite library by using 2 dinucleotide repeats. The microsatellite markers were tested for cross-amplification in O.

Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization

C. A. Polido, Mantello, C. C., Moraes, A. P., Souza, A. P., and Forni-Martins, E. R., “Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization”, vol. 13, pp. 10390-10397, 2014.

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata.

Characterization of nine microsatellite loci for the tree species Parapiptadenia rigida

B. F. Costa, Rodrigues, L. A., Ruas, E. A., Souza, L. B., Ruas, C. F., Vieira, B. G., Conson, A. R. O., and Ruas, P. M., “Characterization of nine microsatellite loci for the tree species Parapiptadenia rigida”, vol. 11. pp. 2338-2342, 2012.

Parapiptadenia rigida, locally known as angico, is a tropical tree common in the semideciduous Brazilian forest. Its wood is naturally resistant to insect attack and is useful for construction. Extracts from the tree have medicinal properties. We characterized nine microsatellite loci for P. rigida. Thirty-five alleles were detected in a sample of 45 individuals from 3 different populations, with an average of 3.9 alleles per locus. The average polymorphic information content ranged from 0.099 to 0.640.

Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species

G. M. Guimarães-Marques, Batista, J. S., Guimarães, H. M., Naice-Daou, M. V., Lima, M. P., Formiga, K. M., Santos, J. M. M., Lima, C. A. P., and Rafael, M. S., “Isolation and characterization of 25 microsatellite DNA loci for Anopheles albitarsis sensu lato and inter-specific amplification in 5 congeneric species”, vol. 11, pp. 4198-4203, 2012.

The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium.

Isolation and characterization of 8 microsatellite loci from Chrysophyllum gonocarpum (Sapotaceae)

L. A. Rodrigues, Ruas, E. A., Signori, N. C., Costa, B. F., Farias-Missão, D. C. L., Vieira, B. G., Conson, A. R. O., Chaves, C. L., Delfini, J., Ruas, C. F., and Ruas, P. M., “Isolation and characterization of 8 microsatellite loci from Chrysophyllum gonocarpum (Sapotaceae)”, vol. 12, pp. 416-419, 2013.

Chrysophyllum gonocarpum is a tropical tree species that is very important in the recovery of heterogeneous forests and of degraded areas of permanent preservation. We identified microsatellite loci for C. gonocarpum to assess the genetic variability and the patterns of the population structure of the species. We isolated 8 microsatellite primers by using CT- and GT-enriched genomic libraries. We detected 2-4 alleles with 2.9 alleles per locus on average, by polymerase chain reaction.

Development and characterization of polymorphic microsatellite markers for Chinese raccoon dog (Nyctereutes procyonoides procyonoides)

S. Q. Yan, Li, Y. M., Bai, C. Y., Ding, X. M., Li, W. J., Hou, J. N., Zhao, Z. H., and Sun, J. H., “Development and characterization of polymorphic microsatellite markers for Chinese raccoon dog (Nyctereutes procyonoides procyonoides)”, vol. 12, pp. 6351-6355, 2013.

Chinese raccoon dog (Nyctereutes procyonoides procyonoides) is one of the most important fur-bearing animal species. Information about the genetic background of farmed Chinese raccoon dogs is limited. In this study, 17 polymorphic microsatellite markers were isolated and identified from an (AC)n-microsatellite-enriched library of Chinese raccoon dogs. The number of alleles per locus ranged from 2 to 8 based on 48 individuals tested.

Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici

A. M. Olivatti, Boni, T. A., Silva-Júnior, N. J., Resende, L. V., Gouveia, F. O., and Telles, M. P. C., “Heterologous amplification and characterization of microsatellite markers in the Neotropical fish Leporinus friderici”, vol. 10. pp. 1403-1408, 2011.

Leporinus friderici, native to the Amazon Basin and popularly known as “piau-três-pintas”, has great ecological and economic importance; it is widely fished and consumed throughout much of tropical South America. Knowledge of the genetic diversity of this native species is important to support management and conservation programs. We evaluated microsatellite loci amplification, using heterologous primers, in 31 individuals of L. friderici.

Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species

S. Q. Xuan, Zheng, J. Y., Wang, H., Gao, P., Zhou, S. B., Liu, K., and Zhu, G. - P., “Polymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species”, vol. 10, pp. 3141-3145, 2011.

Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci from 21 L. radiata individuals of a natural population from Yellow Mountain in Anhui Province, China. The number of alleles ranged from two to nine.

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