A COMPREHENSIVE HPLC METHOD FOR MEASURING ABAMECTIN, CHLORANTRANILIPROLE, AND DELTAMETHRIN IN HUMAN PLASMA: DEVELOPMENT, VALIDATION, AND COMPARATIVE EVALUATION

Abstract

Purpose: Pesticides play a critical role in preventing vector-borne diseases, safeguarding crops, and food preservation. Still, the exposure poses significant risks to people and the environment. Thus, an accurate analytical method to detect and quantify this hazardous compound in biological matrices is required. This work is intended to provide an analytical method for the evaluation of Abamectin, Chlorantraniliprole, and Deltamethrin using High-Performance Liquid Chromatography and UV-visible spectrophotometry.

Method: Initially, the pesticides were identified based on their physical properties, melting point, ultraviolet spectroscopy, and the Fourier transform infrared. Further, the HPLC method was developed and validated as per FDA guidelines. The HPLC method involves preparing a standard stock solution of pesticides in acetonitrile: water, making serial dilutions of the stock solution for calibration over a range of linear curve.

Result: The physical identity of the compounds was established as to its purity. The linearity of the data is graphically represented, based on which a strong correlation was reported. Accuracy and precision were done with Quality control samples in different concentrations to get the standard deviation and percentage relative standard deviation. Recovery study results are 60.47-61.87% for abamectin, 54.74-66.32% for chlorantraniliprole, and 49.87-53.86% for deltamethrin. Limits of detection and Limit of quantification are abamectin 0.0126 & 0.0381 ppm; Chlorantraniliprole 0.019 & 0.057 ppm and Deltamethrin 0.023 & 0.07 ppm which shows a high sensitivity of the method.

Conclusion: The HPLC method developed must be precise, as precise as possible composite for the routine quantification of pesticides in various samples.

Novelty: This experiment intends to establish a novel High-Performance Liquid Chromatography method in FDA guidelines for determining Abamectin, Chlorantraniliprole, and Deltamethrin together. This HPLC validated drug usage method is quite sensitive and consistent. This method is acceptable as it includes Ultraviolet Fourier transform infrared and HPLC confirmation, with findings below parts per million. This work is a good activity for a routine study using an innovative mechanism and sophistication that aids in simple laboratory procedures.