L.S. Lima, K.P. Gramacho, N. Carels, R. Novais, F.A. Gaiotto, U.V. Lopes, A.S. Gesteira, H.A. Zaidan, J.C.M. Cascardo, J.L. Pires, F. Micheli
Published: July 14, 2009
Genet. Mol. Res. 8 (3) : 799-808
DOI: https://doi.org/10.4238/vol8-3gmr603

Cite this Article:
L.S. Lima, K.P. Gramacho, N. Carels, R. Novais, F.A. Gaiotto, U.V. Lopes, A.S. Gesteira, H.A. Zaidan, J.C.M. Cascardo, J.L. Pires, F. Micheli (2009). Single nucleotide polymorphisms from Theobroma cacao expressed sequence tags associated with witches’ broom disease in cacao. Genet. Mol. Res. 8(3): 799-808. https://doi.org/10.4238/vol8-3gmr603

About the Authors
L.S. Lima, K.P. Gramacho, N. Carels, R. Novais, F.A. Gaiotto, U.V. Lopes, A.S. Gesteira, H.A. Zaidan, J.C.M. Cascardo, J.L. Pires, F. Micheli
Corresponding Author
F. Micheli
Email: fabienne.micheli@cirad.fr

ABSTRACT

In order to increase the efficiency of cacao tree resistance to witches’ broom disease, which is caused by Moniliophthora perniciosa (Tricholomataceae), we looked for molecular markers that could help in the selection of resistant cacao genotypes. Among the different markers useful for developing marker-assisted selection, single nucleotide polymorphisms (SNPs) constitute the most common type of sequence difference between alleles and can be easily detected by in silico analysis from expressed sequence tag libraries. We report the first detection and analysis of SNPs from cacao-M. perniciosa interaction expressed sequence tags, using bioinformatics. Selection based on analysis of these SNPs should be useful for developing cacao varieties resistant to this devastating disease.

Key words: Single nucleotide polymorphisms, Expressed sequence tags, Bioinformatics, Witches’ broom disease, Single nucleotide polymorphisms,     Witches’ broom disease.