Gao, J.-L., Nie, Y., & Ding, X.-P.
Genet. Mol. Res. 10 (3), 1884-1890 (2011)
Published: August 26, 2011
DOI: http://dx.doi.org/10.4238/vol10-3gmr1391

About the Authors:

Gao, J.-L., Nie, Y., & Ding, X.-P.
Corresponding author: X.-P. Ding
E-mail: brainding@scu.edu.cn

ABSTRACT

This study evaluates the accuracy and cost-effectiveness of a modified primed in situ labeling (PRINS) technique for detecting single-copy genes in male sterility caused by deletion of SRY and DAZ. Peripheral blood samples from 50 healthy men were collected, and medium-term cultured lymphocytes were suspended in fixative solution and spread on clean slides. Using four primers homologous to unique regions of the SRY and DAZ regions of the human Y-chromosome, we enhanced polymerase specificity and hybridization signal. PRINS of SRY and DAZ produced bands at Yp11.3 and Yq11.2, respectively, in all 50 metaphase spreads. PRINS SRY signals were as distinct as those obtained using traditional fluorescence in situ hybridization (FISH), suggesting PRINS as a cost-effective alternative for rapid localization of single-copy genes or small DNA segments. Further improvements may lead to wider adoption in medical genetics.

Keywords: PRINS, FISH, Single-copy gene, SRY, DAZ