Isolation and characterization of microsatellite loci in the fish Coilia mystus (Clupeiformes:Engraulidae) using PCR-based isolation of microsatellite arrays

Yang, J.-Q., Zhou, X.-D., Liu, D., Liu, Z.-Z., & Tang, W.-Q.
Genet. Mol. Res. 10 (3), 1514-1517 (2011)
Published: July 25, 2011
DOI: 10.4238/vol10-3gmr1420

About the Authors

Yang, J.-Q., Zhou, X.-D., Liu, D., Liu, Z.-Z., & Tang, W.-Q.
Corresponding author: W.-Q. Tang

Abstract: Coilia mystus is the most important harvested fish species in China, inhabiting different water environments during its life stages. Population decline of C. mystus has occurred due to overharvesting and water pollution. In this study, eight microsatellite loci were developed for conservation genetics studies in C. mystus. These markers were tested in 20 individuals from the Min River in ChangLe. The number of alleles ranged from 3 to 8, expected heterozygosity from 0.621 to 0.853, and observed heterozygosity from 0.473 to 0.800. Only two loci deviated significantly from Hardy-Weinberg expectations due to heterozygote deficiency. These primers offer a tool for understanding the demography and population structure of this economically important and threatened species.

Keywords: Microsatellite, Conservation, PIMA, Coilia mystus, RAPD-PCR enrichment

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