Association between a single nucleotide polymorphism in the bovine chemerin gene and carcass traits in Qinchuan cattle

W.Q. Tian1, H.C. Wang1, F.B. Song1, L.S. Zan1,2, H. Wang1, H.B. Wang1,2, Y.P. Xin1, and J.A. Ujan1

1College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, P.R. China
2National Beef Cattle Improvement Centre, Yangling, Shaanxi, P.R. China

Corresponding author: L.S. Zan
Genet. Mol. Res. 10 (4): 2833-2840 (2011)
Published November 17, 2011


Qinchuan is a red or yellow draft and beef breed in China. In order to identify a predictor of carcass traits on the basis of associations between carcass traits and gene polymorphism, variation in the bovine chemerin gene was investigated using PCR-single-strand conformational polymorphism and DNA sequencing. An SNP of A868G located in exon 2 of the Bos taurus chemerin gene was detected in 716 samples of six breeds (Jiaxian red, Luxi, Nanyang, Qinchuan, Simmental, and Luxi crossbred steers, and Xia’nan), all in China, and three genotypes (AA, AG, and GG) were found. Based on the χ2 test, the AA/AG/GG genotype frequencies of all six breeds were found to be in Hardy-Weinberg equilibrium. A possible association of A868G with some carcass traits was investigated in 106 Qinchuan cattle. Animals with the AG genotype were found to have significantly lower mean loin eye area and meat tenderness compared to those with the AA and GG genotypes. However, there was no significant association between any individual haplotype and backfat thickness, water holding capacity, or marbling score. We suggest that A868G could be used as a molecular marker in marker-assisted selection for carcass traits.

Key words: Bos taurus; Chemerin gene; PCR-SSCP; SNP; Carcass traits.

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