Simplified preparation of a DNA ladder using PCR

T.-Y. Wang, L. Wang and F. Wang
Genet. Mol. Res. 10 (3): 1631-1635 (2011)
Published August 5, 2011

About the Authors:
T.-Y. Wang, L. Wang and F. Wang
Corresponding author: T.-Y. Wang


Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by extraction with phenol/chloroform, precipitation with ethanol and mixing. Fragments of 100- to 1000-bp DNA were successfully amplified; the sequences showed 100% identity with lambda DNA. This prepared DNA marker displayed clear bands, indicating that it can be used for molecular studies.

Key words: DNA; Ladder; λ DNA; Polymerase chain reaction.

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