D.A. Vela-Vásquez A.M. Sifuentes-Rincón I. Delgado-Enciso W. Arellano-Vera P. Ambriz-Morales V. Treviño-Alvarado
Published: March 06, 2024
Genet. Mol. Res. 23(1): GMR19169DOI: https://doi.org/10.4238/gmr19169

Cite this Article:
D.A. Vela-Vásquez, A.M. Sifuentes-Rincón, I. Delgado-Enciso, W. Arellano-Vera, P. Ambriz-Morales, V. Treviño-Alvarado (2024). Expression analysis of genes related to lipid metabolism in peripheral blood lymphocytes of volunteers under diet intervention including beef with different fatty acid composition. Genet. Mol. Res. 23(1): GMR19169. https://doi.org/10.4238/gmr19169

About the Authors

D.A. Vela-Vásquez A.M. Sifuentes-Rincón I. Delgado-Enciso W. Arellano-Vera P. Ambriz-Morales V. Treviño-Alvarado

ABSTRACT

Beef fatty acid content and composition have an important effect on lipid metabolism; however, gene expression changes in response to their consumption have not been entirely explored. Thirteen candidate genes were selected according to its role in lipid metabolism and confirmed expression in PBMCs. They were studied to determine their gene expression effect in response to two types of beef: Wagyu-Cross beef characterized by a high concentration of total fat with higher concentration of monounsaturated fatty acids (MUFAs) especially oleic acid than Commercial beef. Both types of beef were consumed by two groups of volunteers (120 g commercial or Wagyu-Cross beef, three times per week) during a two-week clinical trial. The hypothesis of this work was: a dietary intervention involving the consumption of beef with different FA concentrations and compositions (Commercial and Wagyu-Cross beef) will lead to changes in the expression patterns of genes associated with total cholesterol, low-density lipoprotein (LDL), high-density lipoprotein, and triglyceride concentrations in blood mononuclear cells obtained from both groups of consumers. Volunteers who consumed commercial beef showed a significant decrease in scavenger receptor class B member 1 (SCARB1), lactamase beta (LACTB), and sorting nexin 13 (SNX13). The expression of SNX13 was inversely correlated with the change that occurred in the volunteers’ LDL level (r= -0.398, P= 0.044) and had a significant predictive value for a decrease in LDL levels, with an area under the curve of 0.750 (P=0.009); thus, SNX13 is proposed as a candidate gene for evaluating changes in LDL concentrations. It is inferred that Wagyu-Cross beef produced a lower expression effect and favorable lipid profile on this consumer- group, due to the PUFAs-mediated SREBP transcription factor-nuclear abundance suppression, resulting in a reduction in cholesterol and FAs synthesis.

Key words:
Beef, Food intervention, Gene expression, Lipid metabolism, MUFAs.