J. Kumchai, Y.-C. Wei, C.-Y. Lee, F.-C. Chen, S.-W. Chin
Published: March 13, 2013
Genet. Mol. Res. 12 (1) : 755-764
DOI: https://doi.org/10.4238/2013.March.13.4
Cite this Article:
J. Kumchai, Y.C. Wei, C.Y. Lee, F.C. Chen, S.W. Chin (2013). Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum. Genet. Mol. Res. 12(1): 755-764. https://doi.org/10.4238/2013.March.13.4
About the Authors
J. Kumchai, Y.-C. Wei, C.-Y. Lee, F.-C. Chen, S.-W. Chin
Corresponding Author
S.-W. Chin
Email: swchin@mail.npust.edu.tw
ABSTRACT
Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed to be true interspecific hybrids. Eighteen and 14 bands from 7 RAPD and 14 ISSR primers, respectively, were polymorphic and present in all five hybrid seedlings and their parents. The morphological characteristics of leaf margin, inflorescence type and spine positions of the five seedlings were intermediate to the parents. These interspecific hybrids had low pollen viability, probably due to abnormal meiosis.
Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed to be true interspecific hybrids. Eighteen and 14 bands from 7 RAPD and 14 ISSR primers, respectively, were polymorphic and present in all five hybrid seedlings and their parents. The morphological characteristics of leaf margin, inflorescence type and spine positions of the five seedlings were intermediate to the parents. These interspecific hybrids had low pollen viability, probably due to abnormal meiosis.
Key words: Interspecific hybrids, Morphological trait, RAPD, ISSR, Solanum melongena, Solanum torvum.