Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)

R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He
Published May 18, 2010
Genet. Mol. Res. 9 (2): 931-934 (2010)
DOI 10.4238/vol9-2gmr806

About the authors
R.X. Wang, T.J. Xu, Y.N. Sun and G.Y. He

Corresponding author
G.Y. He
E-mail: hegy@hust.edu.cn


Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three loci deviated from Hardy-Weinberg equilibrium, and linkage disequilibrium between five pairs of loci was significant. These polymorphic microsatellite loci can be used for genetic diversity analysis and molecular-assisted breeding of M. miiuy.

Key words: Miichthys miiuy; Microsatellite; Molecular marker

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