Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny

N.M.A. Nassar, P.T.C. Gomes, A.M. Chaib, N.N. Bomfim, R.C.D. Batista, R.G. Collevatti
Published: November 03, 2009
Genet. Mol. Res. 8 (4) : 1323-1330
DOI: https://doi.org/10.4238/vol8-4gmr674

Cite this Article:
N.M.A. Nassar, P.T.C. Gomes, A.M. Chaib, N.N. Bomfim, R.C.D. Batista, R.G. Collevatti (2009). Cytogenetic and molecular analysis of an apomictic cassava hybrid and its progeny. Genet. Mol. Res. 8(4): 1323-1330. https://doi.org/10.4238/vol8-4gmr674

About the Authors
N.M.A. Nassar, P.T.C. Gomes, A.M. Chaib, N.N. Bomfim, R.C.D. Batista, R.G. Collevatti

Corresponding author
N.M.A. Nassar
E-mail: nagnassa@rudah.com.br

ABSTRACT

An interspecific hybrid between cassava and Manihot glaziovii acquired an apomixis gene from the parent M. glaziovii. This hybrid was exposed to open pollination during three subsequent generations. Seven sibs and the maternal progenitor of the fourth generation were genotyped using six microsatellite loci previously developed for cassava. All sibs were identical with each other and with their maternal progenitor. Sibs of selfed M. glaziovii proved to be identical when examined with these microsatellite loci. The chromosome complement of the apomictic clone was 2n = 38. We observed multi-embryonic aposporic embryo sacs.

Key words: interspecific hybrid, Apomixis, Wild species, Aneuploidy.

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