J.T. Arruda
Published: August 04, 2009
Genet. Mol. Res. 8 (3) : 938-938
DOI: https://doi.org/10.4238/vol8-3ta021
Cite this Article:
J.T. Arruda (2009). Occurrence of mutations in loci linked to Y chromosome in the offspring born to individuals exposed to ionizing radiation. Genet. Mol. Res. 8(3): 938-938. https://doi.org/10.4238/vol8-3ta021
About the Authors
J.T. Arruda
In September 1987, in Goiânia, GO, Brazil, one of the most serious radiological accidents occurred at a radiotherapy unit involving a source of cesium-137. An area of 2000 m2 wascontaminated and 249 people were exposed, both externally and internally, to substantial dosesof ionizing radiation, resulting in four fatalities due to acute radiation syndrome. The currentstudy examined the occurrence of possible mutations on the Y chromosome of the exposed menand their male offspring divided into two groups: A) eight accidentally exposed men and eight boys; B) twelve occupationally exposed men and sixteen boys; the control group was constitutedby 8 men and 8 boys not exposed. DNA was isolated from peripheral blood lymphocytes and30 loci (SRY, AMELY, ZFY, AZFa-Prox1, SY83, AZFa-Prox2, SY86, SY85, SY84, USP9Y,SY87, DBY, AZFa-Dist1, 12f2, AZFa-Dist2, UTYpe, SY106, SY124, SY127, SY134, SY135,SY143, SY1197, SY1291, SY1125, SY1054, YDAZ3, SY254, SY255, and RH65618) were amplified by the polymerase chain reaction. All DNA tests had a probability of paternity of at least 99.99%. Read more. . . .
Key words: Recombination, Y chromosome, Ionizing radiations, Cesium-137, Y chromosome.