L. Cifuentes, H. Jorquera, M. Acuña, J. Ordóñez, A.L. Sierra
Published: March 18, 2008
Genet. Mol. Res. 7 (1) : 271-275
DOI: https://doi.org/10.4238/vol7-1gmr368
Cite this Article:
L. Cifuentes, H. Jorquera, M. Acuña, J. Ordóñez, A.L. Sierra (2008). Allele frequencies for 12 autosomal short tandem repeat loci in two bolivian populations. Genet. Mol. Res. 7(1): 271-275. https://doi.org/10.4238/vol7-1gmr368
About the Authors
L. Cifuentes, H. Jorquera, M. Acuña, J. Ordóñez, A.L. Sierra
Corresponding author
L. Cifuentes
E-mail: lcifuent@med.uchile.c
ABSTRACT
Two hundred and sixty unrelated subjects who asked for paternity testing at two Bolivian Laboratories in La Paz and Santa Cruz were studied. The loci D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820, TH01, TPOX, and CSF1PO were typed from blood samples, amplifying DNA by polymerase chain reactions and electrophoresis. Allele frequencies were estimated by simple counting and the unbiased heterozygosity was calculated. Hardy-Weinberg equilibrium was studied and gene frequencies were compared between the two samples.
All loci conformed to the Hardy-Weinberg law and allele frequencies were similar in samples from the two cities. The Bolivian gene frequencies
estimated were significantly different from those described for Chile and the United States Hispanic-Americans for most of the loci.
Key words: DNA typing, Population genetics, Forensic science, Short tandem repeat, Allele frequencies, Bolivian population, Short tandem repeat.