Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes

Kleber Agari Campos, Carmem Silvia Fontanetti
Published December 21, 2005
Genet. Mol. Res. 4 (4): 765-770 (2005)

About the Authors
Kleber Agari Campos, Carmem Silvia Fontanetti

Corresponding author
K.A. Campos


Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.

Key words: Diplopoda, Cytogenetics, Heterochromatin, Fluorochromes.

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