Adriana Mimbacas, Francisco Pérez-Bravo, Pedro C. Hidalgo, Gerardo Javiel, Carmen Pisciottano, Rosario Grignola, Ana María Jorge, Juan Pablo Gallino, Jackeline Gasagoite, Horacio Cardoso
Published: February 06, 2003
Genet. Mol. Res. 2 (1) : 29-35

Cite this Article:
A. Mimbacas, F. Pérez-Bravo, P.C. Hidalgo, G. Javiel, C. Pisciottano, R. Grignola, A.María Jorge, J.Pablo Gallino, J. Gasagoite, H. Cardoso (2003). Association between diabetes type 1 and DQB1* alleles in a case-control study conducted in Montevideo, Uruguay. Genet. Mol. Res. 2(1): 29-35.

About the Authors
Adriana Mimbacas, Francisco Pérez-Bravo, Pedro C. Hidalgo, Gerardo Javiel, Carmen Pisciottano, Rosario Grignola, Ana María Jorge, Juan Pablo Gallino, Jackeline Gasagoite, Horacio Cardoso

Corresponding author
A.Mimbacas
E-mail: abmg@iibce.edu.uy

ABSTRACT

We studied HLA DQB1 allele frequencies and the relative risk (RR) of various genotypes in 72 type 1 diabetic patients and 40 control individuals in Uruguay. This is a tri-racial (Caucasian, Black and Indo-American) mixed population. The products of the polymerase chain reaction amplifications were hybridized with oligonucleotides by allele-specific oligonucleotide reverse or dot blot methods. Significant differences between these two groups were observed only for allele DQB1*0302 (35%, RR = 7.34, P<0.001). The frequency of the alleles carrying a non-aspartic acid residue at position 57 was significantly higher in the diabetic patients (85 vs 53%, P<0.001). In contrast, the frequency of Asp alleles was negatively associated with type 1 diabetes (RR = 0.20, P<0.001). The genotype DQB1*0302/DQB1*0201 (33%, RR = 5.41, P<0.05) was positively associated with this disease. The genotype frequencies associated with type 1 diabetes in our population were significantly different from what is known for Caucasian and Black populations as well as compared with another admixed population, from Chile.

Key words: Diabetes type 1, Human leukocyte antigen system, HLA.