Olga Barbosa-Cisneros, Rafael Herrera-Esparza
Published: September 18, 2002
Genet. Mol. Res. 1 (3) : 241-245
Cite this Article:
O. Barbosa-Cisneros, R. Herrera-Esparza (2002). CENP-B is a conserved gene among vegetal. Genet. Mol. Res. 1(3): 241-245.
About the Authors
Olga Barbosa-Cisneros, Rafael Herrera-Esparza
Corresponding author: R. Herrera-Esparza
E-mail: herrerar@cantera.reduaz.mx
ABSTRACT
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a complete human CENP-B gene. In all the plant species, anti-CENP-B antibodies recognized an 80-kDa protein. A 360-bp sequence encoding for the amino terminus region of the CENP-B protein was amplified by PCR in all the species and the nick-translated pG/CNPB probe hybridized with the PCR products. Apparently the CENP-B centromere protein or an equivalent protein is widely distributed in the vegetal kingdom.
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a complete human CENP-B gene. In all the plant species, anti-CENP-B antibodies recognized an 80-kDa protein. A 360-bp sequence encoding for the amino terminus region of the CENP-B protein was amplified by PCR in all the species and the nick translated pG/CNPB probe hybridized with the PCR products. Apparently the CENP-B centromere protein or an equivalent protein is widely distributed in the vegetal kingdom.
Keywords: Centromere formation, CENP-B gene, CENP-B protein, DNA isolation, Vegetal species.