Ricardo Moratelli, Cláudio de Moraes Andrade, Jorge Luís Azevedo de Armada.
Published: May 15, 2002
Genet. Mol. Res. 1 (2) : 128-130
Cite this Article:
R. Moratelli, Cde Moraes Andrade, J.Luís Azev de Armada (2002). A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies. Genet. Mol. Res. 1(2): 128-130.
About the Authors
Ricardo Moratelli, Cláudio de Moraes Andrade, Jorge Luís Azevedo de Armada.
Corresponding author: R. Moratelli
E-mail: rimoratelli@bol.com.br
ABSTRACT
We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This procedure does not require sacrificing the animals.
We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This procedure does not require sacrificing the animals.
Keywords: Cytogenetics, Cell culture, Bats, Chiroptera.