Toxoplasma gondii

Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene

Z. Y. Li, Song, H. Q., Wang, C. R., Zhu, X. Q., Li, Z. Y., Song, H. Q., Wang, C. R., and Zhu, X. Q., Nucleotide variation in the Toxoplasma gondii micronemal protein 8 gene, vol. 15, p. -, 2016.

Toxoplasma gondii is a successful opportunistic protozoan distributed worldwide, which can infect all vertebrates, leading to serious infection, blindness, and abortion. Micronemal (MIC) proteins are critically important for T. gondii infection, as they participate in various stages of the Toxoplasma life cycle, including invasion and attachment to host cells. MIC8 secretion relies on the concentration of intracellular calcium, and can mediate the invasion of T. gondii by interacting with soluble MIC3.

Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations

H. R. Ning, .L.Wang, J., Qin, S. Y., Huang, S. Y., Lou, Z. L., Hu, L. Y., and Zhu, X. Q., Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations, vol. 14, pp. 8414-8419, 2015.

Toxoplasma gondii, an opportunistic protozoan parasite, infects almost all warm-blooded animals. In this study, we examined the sequence variation in rhoptry protein 20 (ROP20) genes among 18 T. gondii isolates collected from different hosts and geographical regions. Full length ROP20 genes were amplified and sequenced. The results showed that the genes were 1659 bp in length and contained only a single exon, and that the A+T content varied from 46.68 to 47.20% among the 18 strains.

Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities

Z. Y. Li, Chen, J., Lu, J., Wang, C. R., and Zhu, X. Q., Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities, vol. 14, pp. 11403-11409, 2015.

The protozoan parasite Toxoplasma gondii has a worldwide distribution; it can cause serious diseases in humans and almost all other warm-blooded animals. Different genotypes of T. gondii result in different lesions in the same host. T. gondii rhoptry protein 8 (TgROP8) is a major factor of T. gondii acute virulence. We examined sequence variation in the TgROP8 gene among T. gondii isolates from different hosts and geographical localities. The TgROP8 gene was amplified from individual isolates and sequenced.

Low-level sequence variation in Toxoplasma gondii calcium-dependent protein kinases among different genotypes

J. L. Wang, Zhang, N. Z., Huang, S. Y., Xu, Y., Wang, R. A., and Zhu, X. Q., Low-level sequence variation in Toxoplasma gondii calcium-dependent protein kinases among different genotypes, vol. 14, pp. 4949-4956, 2015.

The causative agent of toxoplasmosis, Toxoplasma gondii, can infect virtually all nucleated cell types of warm-blooded animals. In this study, we examined the sequence variation in calcium-dependent protein kinase 2 (CDPK2) genes among 13 T. gondii strains from different hosts and geographical locations. The results showed that the lengths of the complete CDPK2 DNA and cDNA sequences were 3671-3673 and 2136 bp, respectively, and the sequence variation was 0-0.9% among different T. gondii strains.

Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations

J. Chen, Fang, S. F., Zhou, D. H., Li, Z. Y., Liu, G. H., and Zhu, X. Q., Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations, vol. 13, pp. 3356-3361, 2014.

Toxoplasma gondii is an opportunistic protozoan parasite that infects a wide range of animals, including humans. The T. gondii eukaryotic translation initiation factor 4A (eIF4A) protein is expressed in the tachyzoite, but its expression is markedly downregulated in the bradyzoite, and it is therefore considered to be associated with tachyzoite virulence.

Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations

Y. Xu, Zhang, N. Z., Chen, J., Liu, G. H., Xu, Q. M., Zhou, D. H., and Zhu, X. Q., Toxoplasma gondii rhoptry protein 38 gene: sequence variation among isolates from different hosts and geographical locations, vol. 13, pp. 4839-4844, 2014.

Toxoplasma gondii is an obligate intracellular parasite that is able to infect almost all mammalian species, and may lead to toxoplasmosis of the host. In the present study, we examined sequence variation in rhoptry protein 38 (ROP38) genes among T. gondii isolates collected from different hosts and geographical regions. The complete ROP38 gene from 13 T. gondii isolates was amplified and sequenced.

Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations

J. Lu, Zhou, D. H., Chen, J., Zhang, N. Z., Wang, R. A., Weng, Y. B., and Zhu, X. Q., Characterization of the Toxoplasma gondii hsp60 gene sequences from different hosts and geographical locations, vol. 13, pp. 6906-6911, 2014.

The intracellular protozoan Toxoplasma gondii is one of the most successful parasites, with the ability to invade all warm-blooded animals, including humans. T. gondii heat shock protein 60 (TgHSP60) plays an important role in intracellular survival and in the differentiation of the parasite, and is also recognized as being associated with its virulence. In the present study, we examined sequence variation in the hsp60 coding region among five T.

Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii

N. Z. Zhang, Huang, S. Y., Zhou, D. H., Xu, Y., He, J. J., and Zhu, X. Q., Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii, vol. 13, pp. 10669-10677, 2014.

Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very important. In this study, we identified a novel putative calcium-dependent protein kinase of T.

Toxoplasma gondii: cloning, sequencing, expression, and antigenic characterization of ROP2, GRA5 and GRA7

M. Igarash, Kano, F., Tamekuni, K., Kawasaki, P. M., Navarro, I. T., Vidotto, O., Vidotto, M. C., Machado, R. Z., and Garcia, J. L., Toxoplasma gondii: cloning, sequencing, expression, and antigenic characterization of ROP2, GRA5 and GRA7, vol. 7, pp. 305-313, 2008.

Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were cloned into the pET102/D-TOPO® vector which contains thioredoxin and polyhistidine tags at the C- and N-ends, respectively, and is expressed in Escherichia coli BL21(DE-3).

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