RFLP

Polymorphism of the inhibin βA gene and its relationship with superovulation traits in Chinese Holstein cows

W. C. Yang, Li, S. J., Chen, L., and Yang, L. G., “Polymorphism of the inhibin βA gene and its relationship with superovulation traits in Chinese Holstein cows”, vol. 13, pp. 269-275, 2014.

Inhibin is a major regulator of secretion of follicle-stimulating hormone, which is involved in follicular development and regulation of steroidogenesis in females. The objectives of this study were to detect polymorphisms of the bovine inhibin beta-A subunit (INHβA) gene and to evaluate its associations with superovulatory responses in 171 Chinese Holstein cows treated for superovulation.

Comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions

E. A. Haliem and Al-Huqail, A. A., “Comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions”, vol. 12, pp. 6284-6298, 2013.

Protein and DNA polymorphismswere surveyed among seven accessions of wild fenugreek (Trigonellafoenum-graecum L.) to estimate their genetic diversity and relationships. Samples were obtained from diverse ecogeographical areas in Saudi Arabia and Yemen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of seed storage protein showed genetic variations among fenugreek germplasms, both quantitatively and qualitatively, generating a total of 168 polypeptide bands with different molecular weights ranging from 4.5 to 300 kDa.

TP53 Pro47Ser and Arg72Pro polymorphisms and colorectal cancer predisposition in an ethnic Kashmiri population

A. S. Sameer, Shah, Z. A., Syeed, N., Banday, M. Z., Bashir, S. M., Bhat, B. A., and Siddiqi, M. A., “TP53 Pro47Ser and Arg72Pro polymorphisms and colorectal cancer predisposition in an ethnic Kashmiri population”, vol. 9, pp. 651-660, 2010.

Two TP53 gene polymorphisms at codon 47 (TP53 Pro47Ser) and at codon 72 (TP53 Arg72Pro) have been associated with susceptibility to various cancers. We carried out a case-control study and examined the genotype distribution of TP53 Pro47Ser and Arg72Pro single nucleotide polymorphisms (SNPs), using a PCR-RFLP approach, to determine if these two SNPs are risk factors for colorectal cancer (CRC) development and to look for a possible correlation of these two SNPs with clinicopathological variables of CRC.

Risk of colorectal cancer associated with the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in the Kashmiri population

A. S. Sameer, Shah, Z. A., Nissar, S., Mudassar, S., and Siddiqi, M. A., “Risk of colorectal cancer associated with the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism in the Kashmiri population”, vol. 10, pp. 1200-1210, 2011.

Methylenetetrahydrofolate reductase (MTHFR) is a critical enzyme in folate metabolism and is involved in DNA synthesis, DNA repair and DNA methylation. The two common functional polymorphisms of MTHFR, 677 C→T and 1298 A→C, have been shown to impact various diseases, including cancer. The 677 C→T polymorphism has been widely investigated in different cancers and has been implicated as a risk factor for the development of various cancers.

Genetic polymorphism of the glutathione-S-transferase P1 gene (GSTP1) and susceptibility to prostate cancer in the Kashmiri population

Q. Qadri, Sameer, A. S., Shah, Z. A., Hamid, A., Alam, S., Manzoor, S., and Siddiqi, M. A., “Genetic polymorphism of the glutathione-S-transferase P1 gene (GSTP1) and susceptibility to prostate cancer in the Kashmiri population”, vol. 10, pp. 3038-3045, 2011.

Glutathione-S-transferase P1 (GSTP1) is a critical enzyme of the phase II detoxification pathway. One of the common functional polymorphisms of GSTP1 is A→G at nucleotide 313, which results in an amino acid substitution (Ile105Val) at the substrate binding site of GSTP1 and reduces catalytic activity of GSTP1.

Genetic characterization of European-Zebu composite bovine using RFLP markers

E. Perez Marson, Ferraz, J. Bento Ster, Meirelles, F. Vieira, Balieiro, J. César de, Eler, J. Pereira, Figueiredo, L. Gustavo Gi, and Mourão, G. Barreto, “Genetic characterization of European-Zebu composite bovine using RFLP markers”, vol. 4, pp. 496-505, 2005.

A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to determine the structure and the genetic variability of this hybrid herd. The genotypes were determined through PCR, followed by digestion with restriction endonucleases.

Mitochondrial genome differences between the stingless bees Melipona rufiventris and Melipona mondury (Apidae: Meliponini)

G. S. Barni, Strapazzon, R., Guerra, Jr., J. C. V., and Moretto, G., “Mitochondrial genome differences between the stingless bees Melipona rufiventris and Melipona mondury (Apidae: Meliponini)”, vol. 6, pp. 8-14, 2007.

Within the Meliponini, a widely distributed group of stingless bees, Melipona rufiventris has been considered as a single, cohesive species. Recently, analysis of morphological characters led to the splitting of this species into two species, M. mondury and M. rufiventris. The former occurs in the Atlantic Rain Forest ranging from Santa Catarina to Bahia States, while the latter is found in other parts of Brazil. We used PCR + RFLP to identify genetic marker patterns of the mtDNA between these species.

Identification and characterization of polymorphisms at the HSA α1-acid glycoprotein (ORM*) gene locus in Caucasians

C. M. Owczarek, Owczarek, A. L., and Board, P. G., “Identification and characterization of polymorphisms at the HSA α1-acid glycoprotein (ORM*) gene locus in Caucasians”, vol. 1, pp. 96-105, 2002.

Human α₁-acid glycoprotein (AGP) or orosomucoid (ORM) is a major acute phase protein that is thought to play a crucial role in maintaining homeostasis. Human AGP is the product of a cluster of at least two adjacent genes located on HSA chromosome 9. Using a range of restriction endonucleases we have investigated DNA variation at the locus encoding the AGP genes in a group of healthy Caucasians. Polymorphisms were identified using BamHI, EcoRI, BglII, PvuII, HindIII, TaqI and MspI.