RAPD

Populations of Erythrina velutina Willd. at risk of extinction

M. F. V. Melo, Gonçalves, L. O., Rabbani, A. R. C., Álvares-Carvalho, S. V., Pinheiro, J. B., Zucchi, M. I., and Silva-Mann, R., “Populations of Erythrina velutina Willd. at risk of extinction”, vol. 14, pp. 10298-10307, 2015.

The goal of this study was to characterize the structure of two natural populations of the coral tree using RAPD and ISSR markers. The study evaluated all individuals in two different areas in the northeastern region of Brazil: the first was in the riparian area, 10 km x 100 m along the edge of the lower São Francisco River, and the second was in the municipality of Pinhão, in a semiarid region between the municipalities of Neópolis and Santana do São Francisco. We used all the coral trees present in those two areas (37 individuals).

Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy

J. Han, Wang, W. Y., Leng, X. P., Guo, L., Yu, M. L., Jiang, W. B., and Ma, R. J., “Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy”, vol. 13, pp. 32-42, 2014.

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of plant cultivars, from which a cultivar identification diagram (CID) of genotyped plant individuals can be constructed manually.

Genetic variation among quince (Cydonia oblonga Mill.) genotypes sampled from the Coruh valley in Turkey

E. Orhan, Nardemir, G., Agar, G., and Ercisli, S., “Genetic variation among quince (Cydonia oblonga Mill.) genotypes sampled from the Coruh valley in Turkey”, vol. 13, pp. 445-449, 2014.

Turkey has very rich quince genetic resources, and the country currently dominates world quince production. In particular, the northeastern part of the country has notable Cydonia oblonga Mill. germplasm. Authenticating the identity of germplasm resources of C. oblonga Mill. would be of great value for breeding practices. In the present study, genetic variations of 14 C. oblonga Mill. genotypes sampled from the Coruh valley of Turkey were investigated. Ten random primers generated 53 DNA markers.

Genetic characterization and authentication of Dimocarpus longan Lour. using an improved RAPD technique

Z. Q. Mei, Fu, S. Y., Yu, H. Q., Yang, L. Q., Duan, C. G., Liu, X. Y., Gong, S., and Fu, J. J., “Genetic characterization and authentication of Dimocarpus longan Lour. using an improved RAPD technique”, vol. 13, pp. 1447-1455, 2014.

Dimocarpus longan Lour. is an edible and traditional herb in China, commonly referred to as longon. An improved randomly amplified polymorphic DNA (RAPD) protocol was here developed in order to determine the geographical origins of D. longan samples collected from 5 provinces in the southern and southwestern areas of China, including Sichuan, Hainan, Fujian, Guangdong, and Guangxi. Generally, the improved RAPD method generated good fingerprinting of the 5 samples using the selected 17 primers.

Identification and authentication of Rosa species through development of species-specific SCAR marker(s)

K. M. I. Bashir, Awan, F. S., Khan, I. A., Khan, A. I., and Usman, M., “Identification and authentication of Rosa species through development of species-specific SCAR marker(s)”, vol. 13, pp. 4130-4139, 2014.

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is why a reliable fingerprinting method was developed to overcome this problem. The development of molecular markers will enable the identification of Rosa species.

RAPD analysis of the genetic diversity among accessions of Fabaceous forages (Poincianella spp) from the Caatinga

R. F. M. Mendes, Neto, R. B. Araújo, Nascimento, M. P. S. B. C., and Lima, P. S. C., “RAPD analysis of the genetic diversity among accessions of Fabaceous forages (Poincianella spp) from the Caatinga”, vol. 13, pp. 5832-5839, 2014.

Among members of the Fabaceae family, native to the Brazilian Caatinga, the species Poincianella pyramidalis and P. bracteosa exhibit particular potential as forage for cattle, sheep and goats. With the aim of establishing genetic relationships within Poincianella, random amplified polymorphic DNA analysis was performed on eight accessions of P. pyramidalis and two accessions of P.

Molecular and morphological characterization of pomegranate (Punica granatum L.) genotypes sampled from Coruh Valley in Turkey

E. Orhan, Ercisli, S., Esitken, A., and Sengul, M., “Molecular and morphological characterization of pomegranate (Punica granatum L.) genotypes sampled from Coruh Valley in Turkey”, vol. 13, pp. 6375-6382, 2014.

The pomegranate is one of the oldest fruits that are traditionally consumed by the local inhabitants of the Coruh Valley, Turkey. In this study, the molecular and morphological characteristics of 19 promising pomegranate genotypes selected from the Coruh Valley were evaluated. For the morphological evaluation, 22 quantitative fruit characteristics were used. For the molecular evaluation, 47 random amplified polymorphic DNA (RAPD) primers were used for polymerase chain reaction analysis.

Genetic and biochemical differentiation in Vitis vinifera (Kabarcik) populations grown at different altitudes in Coruh Valley

G. Agar, Yildirim, N., and Ercisli, S., “Genetic and biochemical differentiation in Vitis vinifera (Kabarcik) populations grown at different altitudes in Coruh Valley”, vol. 11, pp. 211-220, 2012.

We examined genetic differences of four Vitis vinifera populations (A, B, C, D) including local Kabarcik cultivar grown along an altitude gradient of 800, 900, 1000, and 1150 m above sea level in the Coruh Valley (800 m: A population; 900 m: B population; 1000 m: C population; 1150 m: D population). Leaf samples were used for both RAPD and fatty acid analysis. A total of 60 individuals with 15 individuals per population were included in this study. RAPD analyses showed various band sizes, which ranged from 250 to 3000 bp.

A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content

J. H. Amorim, Vidal, R. O., Lacerda-Junior, G. V., Dias, J. C. T., Brendel, M., Rezende, R. P., and Cascardo, J. C. M., “A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content”, vol. 11, pp. 182-189, 2012.

Landfarm soils are employed in industrial and petrochemical residue bioremediation. This process induces selective pressure directed towards microorganisms capable of degrading toxic compounds. Detailed description of taxa in these environments is difficult due to a lack of knowledge of culture conditions required for unknown microorganisms. A metagenomic approach permits identification of organisms without the need for culture.

Development of a species-specific sequence-characterized amplified region marker for roses

S. Riaz, Sadia, B., Awan, F. S., Khan, I. A., Sadaqat, H. A., and Khan, I. A., “Development of a species-specific sequence-characterized amplified region marker for roses”, vol. 11, pp. 440-447, 2012.

DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid with EcoRI confirmed the cloning of GLI-2₇₆₂ in pTZ57R/T.