H.S. Zhu, J.X. Du, Y.Y. Wang, L.F. Wang, L.Q. Hang, G.Y. Yang, Y.L. Wang
Published: April 12, 2011
Genet. Mol. Res. 10(2): 615-620
DOI: https://doi.org/10.4238/vol10-2gmr1086
Cite this Article:
H.S. Zhu, J.X. Du, Y.Y. Wang, L.F. Wang, L.Q. Hang, G.Y. Yang, Y.L. Wang (2011). Prolactin-releasing peptide mRNA expression in mouse medulla remains relatively stable during pregnancy and lactation. Genet. Mol. Res. 10(2): 615-620. https://doi.org/10.4238/vol10-2gmr1086
About the Authors
H.S. Zhu, J.X. Du, Y.Y. Wang, L.F. Wang, L.Q. Hang, G.Y. Yang, Y.L. Wang
Corresponding Author: H.S. Zhu
Email: zhuheshui@163.com
ABSTRACT
We compared levels of prolactin-releasing peptide (PrRP) mRNA expression in mouse medulla at different stages of pregnancy and lactation. Mouse medulla samples were collected on days 6, 12 and 18 of pregnancy and lactation, respectively (six per group), for mRNA. Expression levels of PrRP mRNA in the medulla were measured by semi-quantitative RT-PCR, with glyceraldehyde 3-phosphate dehydrogenase as a control. PrRP mRNA was highly expressed in mouse medulla oblongata on day 6 of pregnancy (0.53), followed by 0.43 at lactation day 6, and 0.42 at lactation day 12. The expression level of PrRP mRNA on days 12 and 18 of pregnancy and day 18 of lactation shared the same value of 0.36. PrRP mRNA levels during lactation decreased slightly compared with that during pregnancy, but the differences between them were not significant. In summary, PrRP mRNA levels in the medulla oblongata remain relatively stable during pregnancy and lactation. This is evidence that medulla PrRP is not involved in the regulation of prolactin secretion.
Key words: PrRP, Mouse, Pregnancy, Lactation.