Population genetic structure

Microsatellite marker analysis reveals the distinction between the north and south groups of hard clam (Meretrix meretrix) in China

X. F. Gu, Dong, Y. H., Yao, H. H., Zhou, X. L., Qi, X. Y., and Lin, Z. H., Microsatellite marker analysis reveals the distinction between the north and south groups of hard clam (Meretrix meretrix) in China, vol. 14, pp. 1210-1219, 2015.

Meretrix meretrix is one of the important commercial bivalves in China. A total of 198 individual clams were collected from 5 locations characteristic of the clam’s 5 main natural habitats in China, that is, Shandong, Jiangsu, Fujian, Guangdong, and Guangxi. Ten polymorphic microsatellite markers were selected to examine the genetic diversity and identify genetic differences between the 5 populations. A total of 183 alleles across 10 loci were detected in the individual clams.

Selected representative microsatellite loci for genetic monitoring and population structure analysis of miniature swine

C. Wang, Xu, L. L., Wu, Y. H., Du, X. Y., Huo, X. Y., Gao, J., Li, Z. K., Li, Y. C., and Chen, Z. W., Selected representative microsatellite loci for genetic monitoring and population structure analysis of miniature swine, vol. 14, pp. 3910-3920, 2015.

To optimize the combination of microsatellite loci for genetic control of outbred swine stocks, 32 of 100 loci distributed among almost all chromosomes (except 12) were screened out by 1.5% agarose, 8% polyacrylamide gel and capillary electrophoresis scanning among 3 miniature swine outbred stocks, namely Bama (BM), Guizhou (GZ) and Tibeta (TB). The mean total and effective allele numbers among these stocks were 12.1 and 5.9, respectively.

Population genetic structure and historical demography of the ground beetle Chlaenius costiger in the Tsinling-Dabashan Mountains of central China

L. - N. Su, Li, X. - C., Meng, H. - Z., Gao, X. - Y., Yin, H., and Li, K., Population genetic structure and historical demography of the ground beetle Chlaenius costiger in the Tsinling-Dabashan Mountains of central China, vol. 14, pp. 3579-3589, 2015.

Population genetic structure and demographic history of the ground beetle Chlaenius costiger (Coleoptera: Carabidae) in the Tsinling-Dabashan Mountains of central China were estimated using mitochondrial DNA sequences (Cox1-tRNALeu-Cox2) of 144 individuals from 16 local populations. The high haplotype diversity was accompanied by low nucleotide diversity. Phylogenetic analysis (Bayesian inference) of the 43 haplotypes revealed no phylogeographic structure.

Population structure and genetic variation of the endangered species Elaeagnus mollis Diels (Elaeagnaceae)

J. M. Zhang and Zhang, F., Population structure and genetic variation of the endangered species Elaeagnus mollis Diels (Elaeagnaceae), vol. 14, pp. 5950-5957, 2015.

Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic allozyme loci, the population genetic structure was investigated for three populations representing the taxa and variants.

Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin

M. P. C. Telles, Collevatti, R. G., Braga, R. S., Guedes, L. B. S., Castro, T. G., Costa, M. C., Silva-Júnior, N. J., Barthem, R. B., and Diniz-Filho, J. A. F., Geographical genetics of Pseudoplatystoma punctifer (Castelnau, 1855) (Siluriformes, Pimelodidae) in the Amazon Basin, vol. 13, pp. 3656-3666, 2014.

Geographical genetics allows the evaluation of evolutionary processes underlying genetic variation within and among local populations and forms the basis for establishing more effective strategies for biodiversity conservation at the population level. In this study, we used explicit spatial analyses to investigate molecular genetic variation (estimated using 7 microsatellite markers) of Pseudoplatystoma punctifer, by using samples obtained from 15 localities along the Madeira River and Solimões, Amazon Basin.

Polymorphic microsatellite loci isolated from the yellowbelly threadfin bream, Nemipterus bathybius

X. L. Kong, Chen, Z. Z., Lin, L., Li, C. H., Xu, S. N., and Liu, Y., Polymorphic microsatellite loci isolated from the yellowbelly threadfin bream, Nemipterus bathybius, vol. 13, pp. 5254-5257, 2014.

Twenty-two polymorphic microsatellite loci were isolated and characterized from a (GT)13-enriched Nemipterus bathybius genomic library. The number of alleles per locus ranged from 4 to 13, with an average of 7.86. The observed and expected heterozygosity was 0.167-0.889 and 0.278-0.904, respectively, with averages of 0.590 and 0.690. Three loci deviated from Hardy-Weinberg proportions, and 2 loci showed evidence of null alleles. No significant linkage disequilibrium was detected in the pairwise comparisons among the 22 loci.

Development of polymorphic microsatellite markers and the population genetic structure of the half-fin anchovy, Setipinna taty

Y. N. Sun, Qin, Y., Zhu, Z. H., and Sun, D. Q., Development of polymorphic microsatellite markers and the population genetic structure of the half-fin anchovy, Setipinna taty, vol. 13, pp. 6293-6302, 2014.

Microsatellite markers for the half-fin anchovy Setipinna taty were developed from the enriched (CA)15 genomic library, and they were used for the population genetic studies of the half-fin anchovy from Chinese coastal areas. Samples were collected from five localities of the East China Sea and the Yellow Sea. Eleven simple sequence repeat markers were used to assess genetic differentiation in 30 individuals at each locality. As a result, 59 alleles were recorded over all loci with an average of 5.36 alleles per locus.

Evaluation of genetic diversity and population structure in a commercially important freshwater fish Prochilodus costatus (Characiformes, Prochilodontidae) using complex hypervariable repeats

T. M. Barroca, Santos, G. B., Duarte, N. V. R., and Kalapothakis, E., Evaluation of genetic diversity and population structure in a commercially important freshwater fish Prochilodus costatus (Characiformes, Prochilodontidae) using complex hypervariable repeats, vol. 11, pp. 4456-4467, 2012.

We used complex hypervariable repeats to evaluate the genetic diversity and structure of Prochilodus costatus (Characiformes), an ecologically and economically important species endemic to the São Francisco River basin. Hydroelectric dams along the river have led to population fragmentation, which can limit gene flow. Restocking from hatcheries has been used to repopulate declining populations. To determine how fragmentation and hatchery supplementation affect P.

Genetic variability in Blanco Orejinegro breed cattle populations in Colombia

R. Martínez, Llinás, A. P., and M.-Rocha, J. F., Genetic variability in Blanco Orejinegro breed cattle populations in Colombia, vol. 12, pp. 1083-1094, 2013.

We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number of alleles per marker was 14.2 and ranged from 16 (TGLA126) to 22 (TGLA227).

High genetic differentiation of Aegla longirostri (Crustacea, Decapoda, Anomura) populations in southern Brazil revealed by multi-loci microsatellite analysis

M. L. Bartholomei-Santos, Roratto, P. A., and Santos, S., High genetic differentiation of Aegla longirostri (Crustacea, Decapoda, Anomura) populations in southern Brazil revealed by multi-loci microsatellite analysis, vol. 10, pp. 4133-4146, 2011.

Species with a broad distribution rarely have the same genetic make-up throughout their entire range. In some cases, they may constitute a cryptic complex consisting of a few species, each with a narrow distribution, instead of a single-, widely distributed species. These differences can have profound impacts for biodiversity conservation planning.

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