Polymorphic microsatellite loci

Development and characterization of new microsatellite markers of Fenneropenaeus penicillatus

Y. Yuan, Li, Z. B., Ning, Y. F., Deng, H. W., Shangguan, J. B., Huang, Y. S., and Dai, G., Development and characterization of new microsatellite markers of Fenneropenaeus penicillatus, vol. 14. pp. 6679-6682, 2015.

Thirteen new polymorphic microsatellite markers in Fenneropenaeus penicillatus were isolated and characterized. The polymorphism of the thirteen microsatellite markers was tested by 30 individuals from Lianjiang, China. It showed that the number of al­leles per locus ranged from 3 to 6 and the Polymorphism Information Content (PIC) was from 0.324 to 0.706. The observed and expected heterozygosities were 0.3217-0.8023 and 0.1977-0.6783, respectively. Only one loci (LJ-19) deviated significantly from Hardy-Weinberg equilibrium (HWE) (P 0.00385).

Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota

J. B. Shangguan, Li, Z. B., Ning, Y. F., Huang, Y. S., Yuan, Y., Lu, J., Li, B. B., and Mao, X. Q., Screening and characterization of novel polymorphic microsatellite markers from sea cucumber Holothuria leucospilota, vol. 14, pp. 6555-6560, 2015.

The sea cucumber Holothuria leucospilota has high me­dicinal value and rich nutritional edible value, and thus is a commercially important aquatic product in China. Microsatellite loci were developed and screened using a fast isolation protocol and amplified fragment length polymorphism of sequences containing repeats. In this study, 16 novel polymorphic microsatellite markers in H. leucospilota were identified, and the relevant genetic variability index was assessed using 30 individu­als from a wild population.

Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus

J. B. Shangguan, Li, Z. B., Li, Q. H., Dai, G., and Ning, Y. F., Screening and characterization of new microsatellite markers in Fenneropenaeus penicillatus, vol. 13, pp. 6079-6082, 2014.

Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicillatus were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The polymorphism information content ranged from 0.2703 to 0.7598, and the number of alleles per locus varied from 3 to 6.

Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)

H. Li, Zhou, Z. - S., Guo, J., and Lin, L. H., Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae), vol. 11, pp. 4707-4710, 2012.

We isolated and characterizated 12 polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae). The loci were screened in 37 E. velox individuals. The number of alleles ranged from 6 to 16. The observed heterozygosity ranged from 0.432 to 0.919, and the expected heterozygosity ranged from 0.685 to 0.902. These microsatellite markers should prove useful for population genetic studies of E. velox and other Eremias species.

Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci

F. Bello and Becerra, V., Genetic variability and heterogeneity of Venezuelan equine encephalitis virus vector Ochlerotatus taeniorhynchus (Diptera: Culicidae) populations of the Colombian Atlantic coast, based on microsatellite loci, vol. 8, pp. 1179-1190, 2009.

In Colombia, the mosquito Ochlerotatus taeniorhyn­chus has been identified as an efficient vector of the epidemic-epi­zootic Venezuelan equine encephalitis virus. We evaluated the ge­netic variability and heterogeneity of this mosquito in Colombian populations using eight microsatellite DNA loci. Two hundred and ten mosquito specimens collected from seven populations of the Co­lombian Atlantic coast (San Bernardo del Viento, Coveñas, Carta­gena, Barranquilla, Ciénaga, Dibulla, and Riohacha) were analyzed.

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