Polymorphic

Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy

X. Y. Li, Xu, H. X., and Chen, J. W., Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy, vol. 13, pp. 3384-3394, 2014.

Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products.

Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)

B. Wu, Bai, L. J., Yang, A. G., Zhou, L. Q., and Liu, Z. H., Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae), vol. 13, pp. 10643-10647, 2014.

In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to 0.8954.

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