Obese gene polymorphism in Pietrain and large white pigs after a divergent selection
“Obese gene polymorphism in Pietrain and large white pigs after a divergent selection”, vol. 7, pp. 1217-1222, 2008.
, The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fragment length polymorphism using the restriction enzyme HinfI. The data were statistically analyzed by the chi-square test.