Molecular marker

Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines

M. Ejaz, Qidi, Z., Gaisheng, Z., Qunzhu, W., and Xinbo, Z., Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines, vol. 12, pp. 5207-5214, 2013.

To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta (T.spelta) -90-110 and their maintainer line, A-90-110, at the molecular level.

Development of 1047 insertion-deletion markers for rice genetic studies and breeding

Y. X. Zeng, Wen, Z. H., Ma, L. Y., Ji, Z. J., Li, X. M., and Yang, C. D., Development of 1047 insertion-deletion markers for rice genetic studies and breeding, vol. 12, pp. 5226-5235, 2013.

In this study, a total of 1047 insertion-deletion (InDel) primer pairs distributed across the rice genome were developed and experimentally validated. The primer pairs were designed based on the InDel length polymorphisms between 93-11 (Oryza sativa ssp indica cv.) and Nipponbare (Oryza sativa ssp japonica cv.), aiming for utilization between indica and japonica rice, or between other inter-subspecific rice cultivars.

Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting

H. S. An, Park, J. Y., Myeong, J. I., and An, C. M., Genetic relationships of Pacific abalone (Haliotidae) species determined using universal rice primer-polymerase chain reaction fingerprinting, vol. 12, pp. 6309-6318, 2013.

Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and H. diversicolor diversicolor, whose morphological differentiation is difficult.

Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae)

R. X. Wang, Xu, T. J., Sun, Y. N., and He, G. Y., Polymorphic microsatellite loci from two enriched genomic libraries for the genetic analysis of the miiuy croaker, Miichthys miiuy (Sciaenidae), vol. 9. pp. 931-934, 2010.

Twelve polymorphic microsatellites from the (AG)13 and (CA)13 enriched genomic libraries of Miichthys miiuy were isolated and characterized in a test population; the number of alleles ranged from two to nine. The observed and expected heterozygosities ranged from 0.1923 to 1.0000 and from 0.2633 to 0.8337, respectively. Three loci deviated from Hardy-Weinberg equilibrium, and linkage disequilibrium between five pairs of loci was significant.

Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays)

E. C. Oliveira, Júnior, A. T. Amaral, Gonçalves, L. S. A., Pena, G. F., Júnior, S. P. Freitas, Ribeiro, R. M., and Pereira, M. G., Optimizing the efficiency of the touchdown technique for detecting inter-simple sequence repeat markers in corn (Zea mays), vol. 9, pp. 835-842, 2010.

We evaluated the efficiency of the touchdown method to determine the ideal PCR conditions for distinct inter-simple sequence repeat primers for processing DNA from common corn, popcorn, sweet corn, and a Tripsacum-maize hybrid. Genomic DNA was extracted from eight accessions of corn: two of the dent type, one Tripsacum-maize hybrid, one sweet corn, one flint-type corn, and three popcorn.

Genetic variability of Mahanarva sp (Hemiptera: Cercopidae) collected from different sites in Brazil

A. M. Auad, Domingues, R., Machado, M. A., Souza, L. S., Carvalho, G. S., and Paula-Moraes, S. V., Genetic variability of Mahanarva sp (Hemiptera: Cercopidae) collected from different sites in Brazil, vol. 9. pp. 1005-1010, 2010.

Spittlebugs are the leading cause of damage to tall grasses. Annual losses are estimated to reach 2.1 billion dollars in sugarcane crops and grazing land throughout the world. Correct identification of these species is difficult due to similarities in color, body size and male genitalia. Molecular markers have been useful in the identification and assessment of genetic diversity of many species. We investigated the genetic diversity of the spittlebug species Mahanarva fimbriolata, M. spectabilis and M.

Isolation and characterization of polymorphic microsatellite loci in the hard-shelled mussel, Mytilus coruscus (Mytilidae)

T. - J. Xu, Sun, Y. - N., Yuan, Y. - T., Liao, Z., and Wang, R. - X., Isolation and characterization of polymorphic microsatellite loci in the hard-shelled mussel, Mytilus coruscus (Mytilidae), vol. 9. pp. 1388-1391, 2010.

Mytilus coruscus is one of the most important cultured species of marine shellfish in China. Using an expressed sequence tag-library and two microsatellite-enriched genomic libraries of M. coruscus, we isolated and characterized 12 polymorphic microsatellites in a test population; the number of alleles ranged from three to seven, and the observed and expected heterozygosities ranged from 0.0333 to 0.8571 and from 0.3452 to 0.8267, respectively. Four loci deviated from Hardy-Weinberg equilibrium.

Genetic relationships among Heliconia (Heliconiaceae) species based on RAPD markers

L. P. Marouelli, Inglis, P. W., Ferreira, M. A., and Buso, G. S. C., Genetic relationships among Heliconia (Heliconiaceae) species based on RAPD markers, vol. 9, pp. 1377-1387, 2010.

The family Heliconiaceae contains a single genus, Heliconia, with approximately 180 species of Neotropical origin. This genus was formerly allocated to the family Musaceae, but today forms its own family, in the order Zingiberales. The combination of inverted flowers, a single staminode and drupe fruits is an exclusive characteristic of Heliconia. Heliconias are cultivated as ornamental garden plants, and are of increasing importance as cut flowers.

Genetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers

C. B. M. Cerqueira-Silva, Cardoso-Silva, C. B., Santos, E. S. L., Conceição, L. D. H. C. S., Pereira, A. S., Oliveira, A. C., and Corrêa, R. X., Genetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers, vol. 9, pp. 2123-2130, 2010.

In spite of the importance of and the considerable variability observed in Passiflora (Passifloraceae), little is known about the genetic diversity of most of the species of this genus. We evaluated the genetic diversity by RAPD markers in 18 genotypes of Passiflora trintae. The 15 primers generated 112 markers, 84% of which were polymorphic. The genetic distance estimated by the complement of the Dice index (average dissimilarity = 0.30) and genotype grouping based on the UPGMA algorithm showed low variability among genotypes.

Development and characterization of polymorphic microsatellite markers in the gray mullet (Mugil cephalus)

T. - J. Xu, Sun, D. - Q., Shi, G., and Wang, R. - X., Development and characterization of polymorphic microsatellite markers in the gray mullet (Mugil cephalus), vol. 9, pp. 1791-1795, 2010.

Using an (AG)13 enriched genomic library of Mugil cephalus, 12 polymorphic microsatellite loci were isolated and char­acterized in a test population; the number of alleles ranged from 2 to 11. The observed and expected heterozygosities ranged from 0.2593 to 0.8966 and from 0.3047 to 0.8454, respectively. Two loci deviated from Hardy-Weinberg equilibrium; linkage disequilibrium among the 12 loci was non-significant. These polymorphic micro­satellite loci will be useful for genetic diversity analysis and mole­cule-assisted breeding of the gray mullet.

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