Molecular identification

Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii

T. Lin, Tang, C. G., Li, Q. H., Ji, J., Ge, H. Y., Zhang, X. Y., and Sun, H. P., Identification of aac(2')-I Type b aminoglycoside-modifying enzyme genes in resistant Acinetobacter baumannii, vol. 14, pp. 1828-1835, 2015.

The aim of this study was to investigate the mechanism underlying the drug resistance of Acinetobacter baumannii toward aminoglycosides. A total of 32 A. baumannii strains were identified by molecular identification and subsequently isolated. The isolates were then amplified by polymerase chain reaction to analyze the 9 aminoglycoside-modifying enzyme genes and 7 16S rRNA methylase genes. Five types of aminoglycoside-modifying enzyme genes and 1 type of 16S rRNA methylase gene were detected in the 32 drug-resistant A. baumannii strains.

Molecular characterization of the endophytic fungal community associated with Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) (Pontederiaceae) native to the Upper Paraná River floodplain, Brazil

T. T. Almeida, Orlandelli, R. C., Azevedo, J. L., and Pamphile, J. A., Molecular characterization of the endophytic fungal community associated with Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) (Pontederiaceae) native to the Upper Paraná River floodplain, Brazil, vol. 14, pp. 4920-4931, 2015.

Endophytic fungi live in the interior of healthy plants without causing them any damage. These fungi are of biotechnological interest; they may be used in the biological control of pests and plant diseases, and in the pharmaceutical industry. The aquatic macrophytes Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) belong to the Pontederiaceae family. The first is a fixed-floating species and the second is a free-floating species that is known for its phytoremediation potential. The fungal endophytes associated with the leaves of E.

Novel DNA extraction assay for molecular identification of Aedes spp eggs

M. T. S. Freitas, Gomes-Júnior, P. P., Batista, M. V. A., Leal-Balbino, T. C., Araujo, A. L., and Balbino, V. Q., Novel DNA extraction assay for molecular identification of Aedes spp eggs, vol. 13, pp. 8776-8782, 2014.

Aedes aegypti and A. albopictus represent the two most important species of mosquitoes in relation to dengue virus transmission both in the Americas and Asia. However, the study of theses species generally requires the establishment of a colony for the larvae to hatch, or waiting for the adult development to perform its taxonomic classification, which is time consuming. Thus, the establishment of new methods aimed at obtaining DNA directly from the mosquito eggs is relevant.

Diversity of endophytic fungal community associated with Piper hispidum (Piperaceae) leaves

R. C. Orlandelli, Alberto, R. N., Filho, C. J. Rubin, and Pamphile, J. A., Diversity of endophytic fungal community associated with Piper hispidum (Piperaceae) leaves, vol. 11, pp. 1575-1585, 2012.

Tropical and subtropical plants are rich in endophytic community diversity. Endophytes, mainly fungi and bacteria, inhabit the healthy plant tissues without causing any damage to the hosts. These fungi can be useful for biological control of pathogens and plant growth promotion. Some plants of the genus Piper are hosts of endophytic microorganisms; however, there is little information about endophytes on Piper hispidum, a medicinal shrub used as an insecticide, astringent, diuretic, stimulant, liver treatment, and for stopping hemorrhages.

Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae)

S. A. Rhoden, Garcia, A., Filho, C. J. Rubin, Azevedo, J. L., and Pamphile, J. A., Phylogenetic diversity of endophytic leaf fungus isolates from the medicinal tree Trichilia elegans (Meliaceae), vol. 11, pp. 2513-2522, 2012.

Various types of organisms, mainly fungi and bacteria, live within vegetal organs and tissues, without causing damage to the plant. These microorganisms, which are called endophytes, can be useful for biological control and plant growth promotion; bioactive compounds from these organisms may have medical and pharmaceutical applications. Trichilia elegans (Meliaceae) is a native tree that grows abundantly in several regions of Brazil.

Genetic relationships among five varieties of Curcuma alismatifolia (Zingiberaceae) based on ISSR markers

S. Taheri, Abdullah, T. L., Abdullah, N. A. P., and Ahmad, Z., Genetic relationships among five varieties of Curcuma alismatifolia (Zingiberaceae) based on ISSR markers, vol. 11, pp. 3069-3076, 2012.

The genus Curcuma is a member of the ginger family (Zingiberaceae) that has recently become popular for use as flowering pot plants, both indoors and as patio and landscape plants. We used PCR-based molecular markers (ISSRs) to assess genetic variation and relationships between five varieties of curcuma (Curcuma alismatifolia) cultivated in Malaysia. Sixteen ISSR primers generated 139 amplified fragments, of which 77% had high polymorphism among these varieties.

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