Microsatellites

New microsatellite markers for bananas (Musa spp)

E. P. Amorim, Silva, P. H., Ferreira, C. F., Amorim, V. B. O., Santos, V. J., Vilarinhos, A. D., Santos, C. M. R., Júnior, M. T. Souza, and Miller, R. N. G., “New microsatellite markers for bananas (Musa spp)”, vol. 11. pp. 1093-1098, 2012.

Thirty-four microsatellite markers (SSRs) were identified in EST and BAC clones from Musa acuminata burmannicoides var. Calcutta 4 and validated in 22 Musa genotypes from the Banana Germplasm Bank of Embrapa-CNPMF, which includes wild and improved diploids. The number of alleles per locus ranged from 2 to 14. The markers were considered highly informative based on their polymorphism information content values; more than 50% were above 0.5.

Geographic shifts in climatically suitable areas and loss of genetic variability in Dipteryx alata (“Baru” Tree; Fabaceae)

J. A. F. Diniz-Filho, Collevatti, R. G., Chaves, L. J., Soares, T. N., Nabout, J. C., Rangel, T. F., Melo, D. B., Lima, J. S., and Telles, M. P. C., “Geographic shifts in climatically suitable areas and loss of genetic variability in Dipteryx alata (“Baru” Tree; Fabaceae)”, vol. 11, pp. 1618-1626, 2012.

Many species are expected to suffer strong shifts in their geographic ranges due to climate changes in the next 50 years, with severe consequences for biodiversity patterns and population structure. We used here an ensemble forecast approach for obtaining species’ range in which multiple species distribution models and climatic models were combined to model loss of genetic variability in Baru, Dipteryx alata (Fabaceae), an economically important Neotropical tree native to the Cerrado of Brazil.

Application of functional genomic information to develop efficient EST-SSRs for the chicken (Gallus gallus)

M. R. Bakhtiarizadeh, Arefnejad, B., Ebrahimie, E., and Ebrahimi, M., “Application of functional genomic information to develop efficient EST-SSRs for the chicken (Gallus gallus)”, vol. 11, pp. 1558-1574, 2012.

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional genomics information in recent years has opened new possibilities for the generation of molecular markers.

First isolation and characterization of genomic SSR markers for the giant red shrimp Aristaeomorpha foliacea (Risso, 1827)

R. Cannas, Marcias, S., Sacco, F., Cau, A., and Deiana, A. M., “First isolation and characterization of genomic SSR markers for the giant red shrimp Aristaeomorpha foliacea (Risso, 1827)”, vol. 11, pp. 2745-2748, 2012.

Fourteen microsatellite markers were isolated from the giant red shrimp Aristaeomorpha foliacea (Risso, 1827) using the FIASCO protocol (fast isolation by AFLP of sequences containing repeats). Polymorphism was assessed in 30 individuals from two localities of the western Mediterranean basin (N = 20 from Sardinia and N = 10 from Sicily); nine loci showed polymorphism with 2 to 19 alleles per locus (average: 8.9).

Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis

H. S. An, Lee, J. W., and Park, J. Y., “Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis”, vol. 11, pp. 3904-3922, 2012.

Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species.

Development and characterization of DNA microsatellite primers for buriti (Mauritia flexuosa L.f.)

E. V. Menezes, Souto, W. F. S., Ciampi, A. Y., Azevedo, V. C. R., Valério, H. M., and Pimenta, M. A. S., “Development and characterization of DNA microsatellite primers for buriti (Mauritia flexuosa L.f.)”, vol. 11, pp. 4058-4062, 2012.

Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti that occurs in the Cerrado biome. It is characteristic of the vereda, a typical ecosystem of central Brazil. In this phytophysiognomy, M. flexuosa and other groups of arboreal-herbaceous species develop in open fields with very humid soils. M. flexuosa can be found in forest borders and is a palm tree with a wide distribution in South America (Brazil, Colombia, Venezuela, French Guyana Ecuador, Peru, and Bolivia).

Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations

D. Kaczmarczyk, “Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations”, vol. 12. pp. 154-159, 2013.

Fifteen sets of PCR primers designed for the amplification of microsatellite loci from Danio rerio and Phoxinus neogaeus Campostoma DNA that have been proven applicable for molecular studies of several species of cyprinids were tested for amplification of microsatellites from lake minnow DNA. The samples were taken from 298 fish inhabiting 6 distinct populations located in Poland, and DNA was extracted from dried fin clips.

Diversity and genetic structure among subpopulations of Gossypium mustelinum (Malvaceae)

M. F. Alves, Barroso, P. A. V., Ciampi, A. Y., Hoffmann, L. V., Azevedo, V. C. R., and Cavalcante, U., “Diversity and genetic structure among subpopulations of Gossypium mustelinum (Malvaceae)”, vol. 12, pp. 597-609, 2013.

Gossypium mustelinum is the only cotton species native to Brazil; it is endemic to the semi-arid region of the northeast. The populations are found near perennial and semi-perennial sources of water, such as ponds or pools in intermittent streams. Problems with in situ conservation derive from human interference in its habitat, mainly because of excessive cattle grazing and deforestation. Establishing efficient strategies for in situ conservation requires knowledge of the genetic structure of the populations.

Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing

L. A. Fusinatto, Lopes, S., Silva-Ferreira, A., Alexandrino, J., Haddad, C. F. B., Rocha, C. F. D., and Sequeira, F., “Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing”, vol. 12. pp. 230-234, 2013.

The new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecológica de Guapiaçu, REGUA) located in the central region of the Rio de Janeiro State, in Brazil. The mean number of alleles per locus ranged from 3 to 12.

Microsatellite usefulness is independent of phylogenetic distance in Tyrant flycatchers (Aves: Tyrannidae): a test using two globally threatened species

B. Mahler, Schneider, A. R. R., Di Giacomo, A. S., Di Giacomo, A. G., Reboreda, J. C., and Tiedemann, R., “Microsatellite usefulness is independent of phylogenetic distance in Tyrant flycatchers (Aves: Tyrannidae): a test using two globally threatened species”, vol. 12. pp. 2966-2972, 2013.

Tyrant flycatchers (Aves: Tyrannidae) are endemic to the New World, and many species of this group are threatened or near-threatened at the global level. The aim of this study was to test the 18 microsatellite markers that have been published for other Tyrant flycatchers in the Strange-tailed Tyrant (Alectrurus risora) and the Sharp-tailed Tyrant (Culicivora caudacuta), two endemic species of southern South American grasslands that are classified as vulnerable. We also analyzed the usefulness of loci in relation to phylogenetic distance to the source species.

Pages

Subscribe to Microsatellites