Microsatellite

Characterization of 12 microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae), and cross-amplification in five other lamprey species

E. K. Spice, Whitesel, T. A., McFarlane, C. T., and Docker, M. F., “Characterization of 12 microsatellite loci for the Pacific lamprey, Entosphenus tridentatus (Petromyzontidae), and cross-amplification in five other lamprey species”, vol. 10. pp. 3246-3250, 2011.

The Pacific lamprey (Entosphenus tridentatus) is an anadromous fish that is of conservation concern in North America and Asia. Data on Pacific lamprey population structure are scarce and conflicting, impeding conservation efforts. We optimized 12 polymorphic microsatellite loci for the Pacific lamprey. Three to 13 alleles per locus were observed in a sample of 51 fish collected from the West Fork Illinois River, Oregon. Observed heterozygosity ranged from 0.235 to 0.902 and expected heterozygosity ranged from 0.214 to 0.750.

Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona

M. V. C. Viana, Miranda, E. A., de Francisco, A. K., Carvalho, C. A. L., and Waldschmidt, A. M., “Transferability of microsatellite primers developed for stingless bees to four other species of the genus Melipona”, vol. 10, pp. 3942-3947, 2011.

Microsatellite markers are a useful tool for ecological monitoring of natural and managed populations. A technical limitation is the necessity for investment in the development of primers. Heterologous primers can provide an alternative to searching for new loci. In bees, these markers have been used in populational and intracolonial genetic analyses. The genus Melipona has the largest number of species among bee genera, about 70, occurring throughout the Neotropical region. However, only five species of the genus Melipona have specific microsatellite markers.

Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae)

S. Yang, Wang, L., Zhang, Y., Liu, X. C., Lin, H. R., and Meng, Z. N., “Development and characterization of 32 microsatellite loci in the giant grouper Epinephelus lanceolatus (Serranidae)”, vol. 10. pp. 4006-4011, 2011.

An economically important marine fish species, the giant grouper Epinephelus lanceolatus (Serranidae) is widely cultured in Taiwan and costal areas of China. We isolated and characterized 32 polymorphic microsatellite loci from a CA-enriched genomic library of giant grouper. The number of alleles per locus ranged from 3 to 7, with a mean of 4.69. Observed and expected heterozygosities per locus varied from 0.387 to 1.000 and from 0.377 to 0.843, respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium.

Brazilian Nelore cattle: a melting pot unfolded by molecular genetics

M. A. C. Dani, Heinneman, M. B., and Dani, S. U., “Brazilian Nelore cattle: a melting pot unfolded by molecular genetics”, vol. 7, pp. 1127-1137, 2008.

The aim of the present article was to study the population structure and genetic diversity of Nelore cattle and genetic relationships between Nelore and different taurine and zebu breeds raised in Brazil. DNA polymorphism analysis was carried out with 1976 animals of 16 zebu, taurine and synthetic breeds raised in Brazil. A higher genetic differentiation was observed in taurine than in zebu cattle. Gene flow was intense between the different zebu populations.

Population genetic structure of the blue-fronted Amazon (Amazona aestiva, Psittacidae: Aves) based on nuclear microsatellite loci: implications for conservation

K. C. E. Leite, Seixas, G. H. F., Berkunsky, I., Collevatti, R. G., and Caparroz, R., “Population genetic structure of the blue-fronted Amazon (Amazona aestiva, Psittacidae: Aves) based on nuclear microsatellite loci: implications for conservation”, vol. 7, pp. 819-829, 2008.

The blue-fronted Amazon (Amazona aestiva) is a widely distributed Neotropical parrot and one of the most captured parrots in nature to supply the illegal trade of wild animals. The objectives of the present study were to analyze the genetic structure of A. aestiva to identify management units and support conservation planning and to verified if A. aestiva populations have undergone a recent bottleneck due to habitat loss and capture for the pet trade. The genetic structure was accessed by analyzing six microsatellite loci in 74 individuals of A.

Molecular characterization of wheat germplasm using microsatellite markers

S. Ijaz and Khan, I. A., “Molecular characterization of wheat germplasm using microsatellite markers”, vol. 8, pp. 809-815, 2009.

We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discrimi­nated the accessions and varieties into different clusters; genetic di­versity was the highest between variety Kohistan-97 and accession number 011512, giving a genetic similarity value of 0.4198.

Molecular analysis of apomixis in cassava

N. M. A. Nassar, Kalkmann, D. C., and Collevatti, R., “Molecular analysis of apomixis in cassava”, vol. 5, pp. 487-492, 2006.

Cassava is the main staple for more than 800 million people in the tropics. It is propagated vegetatively by stem cuttings, which maintains superior genotypes but favors disease accumulation and spread. In this report, we present the results of the screening of the progeny and the second generation of the clone UnB 307 for apomixes using microsatellites. A total of 29 plants were screened, representing the maternal plant, its first and second generations that were left to open pollination. About 20% of the offspring were rated as genetically identical plants.

Genetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus

A. B. Grisolia, Moreno, V. R., Campagnari, F., Milazzotto, M. P., Garcia, J. F., Adania, C. H., and Souza, E. B., “Genetic diversity of microsatellite loci in Leopardus pardalis, Leopardus wiedii and Leopardus tigrinus”, vol. 6, pp. 382-389, 2007.

The microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive Leopardus sp. The PCR-amplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and shared alleles were calculated. Sixty-five alleles were found and their sizes ranged from 116 to 216 bp in four microsatellite loci.

Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog

D. C. Salim, Akimoto, A. A., Carvalho, C. B., Oliveira, S. F., Grisolia, C. K., Moreira, J. R., and Klautau-Guimarães, M. N., “Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog”, vol. 6, pp. 348-357, 2007.

The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES.

Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)

J. E. Maldonado, Cosse, M., and González, S., “Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)”, vol. 6, pp. 1118-1122, 2007.

In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847.

Pages

Subscribe to Microsatellite