Microsatellite

Paeonia (Paeoniaceae) expressed sequence tag-derived microsatellite markers transferred to Paeonia delavayi

D. X. Wang, Ma, H., Zhang, Y. L., Duan, A. A., Li, W. J., and Li, Z. H., “Paeonia (Paeoniaceae) expressed sequence tag-derived microsatellite markers transferred to Paeonia delavayi”, vol. 12, pp. 1278-1282, 2013.

Paeonia Franchet contains approximately 30 species of perennial herbs and is the only member of the family Paeoniaceae. Paeonia delavayi is an endangered ornamental plant that has a compound flower color and is endemic to southwest China. Its optimal habitats have been fragmented and its population size has greatly decreased as a result of human activities. Using a modified biotin-streptavidin capture method, 51 primer sets were identified in 3 wild populations of P. delavayi in this study.

Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus

P. - Z. Qi, Xie, C. - X., Guo, B. - Y., Wu, C. - W., Lu, S. - M., and Duan, Y. - J., “Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus”, vol. 12, pp. 1761-1765, 2013.

Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polymorphism information content of 11 loci was more than 0.5 whereas that of the other 4 loci was less than 0.5 but more than 0.25. In addition, the genomes of 30 C.

Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)

V. A. C. Pavinato, Silva-Brandão, K. L., Monteiro, M., Zucchi, M. I., Pinheiro, J. B., Dias, F. L. F., and Omoto, C., “Development and characterization of microsatellite loci for genetic studies of the sugarcane borer, Diatraea saccharalis (Lepidoptera: Crambidae)”, vol. 12. pp. 1631-1635, 2013.

We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the polymorphism information content (ranging from 0.292 to 0.771; 0.535 on average).

Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup)

H. Li, Ge, T. M., Ding, S. X., and Wang, H. J., “Novel tetranucleotide microsatellite markers for Chinese beard eel (Cirrhimuraena chinensis Kaup)”, vol. 12. pp. 2779-2782, 2013.

In this study, we characterized 12 novel polymorphic microsatellite loci for Chinese beard eel (Cirrhimuraena chinensis Kaup) from a tetranucleotide microsatellite-enriched library. Loci screened on a sample of 37 individuals from Xiamen wild stocks revealed 8 to 24 alleles per locus, with a mean of 13.83 over all loci. Observed and expected heterozygosities ranged from 0.270 to 0.944 and 0.439 to 0.942, respectively. These efficient genetic markers thus provide useful tools in the study of the population genetics and phylogeography of Chinese beard eel.

Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)

Q. H. Li, Li, Z. B., Dai, G., Chen, X. J., Chen, L. N., Cao, Y. Y., Shangguan, J. B., and Ning, Y. F., “Characterization of new microsatellite markers of Siganus fuscescens (Siganidae)”, vol. 12, pp. 2751-2754, 2013.

Siganus fuscescens, which is a small commercially important marine fish, is wildly distributed in shallow waters throughout the tropical and subtropical Indo-Pacific and Eastern Mediterranean regions. It is part of a group known as rabbitfish. Fifteen new polymorphic microsatellite markers for S. fuscescens were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 2 to 12, and the polymorphism information content ranged from 0.210 to 0.849.

Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse)

J. Hu, Zhu, X. P., Luo, J., Yin, S. W., Peng, Y. H., Hu, Y. L., and Zhu, F., “Development and characterization of microsatellite loci in a threatened marine fish, Cheilinus undulatus (humphead wrasse)”, vol. 12, pp. 2633-2636, 2013.

Cheilinus undulatus (humphead wrasse) is a marine fish distributed widely throughout the tropical Indo-Pacific. It has been listed as vulnerable in the IUCN Red Data Book and in CITES Appendix II four times. Fifteen microsatellite loci were isolated and characterized for this species. The number of alleles ranged from 3 to 15 per locus, and the observed and expected heterozygosity ranged between 0.0323-0.7742 and 0.2597-0.8773, respectively. The polymorphism information content ranged from 0.2353-0.8520.

Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)

M. P. C. Telles, Silva, J. B., Resende, L. V., Vianello, R. P., Chaves, L. J., Soares, T. N., and Collevatti, R. G., “Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)”, vol. 12, pp. 3124-3127, 2013.

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884.

Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species

P. F. Cruz, Batista, J. S., Formiga, K. M., Rafael, M. S., Tadei, W. P., and Santos, J. M. M., “Isolation and characterization of polymorphic DNA microsatellite loci for Anopheles triannulatus sensu lato (Diptera: Culicidae) and cross-amplification in congeneric species”, vol. 12, pp. 3088-3092, 2013.

Anopheles (Nyssorhynchus) triannulatus is a complex of 3 species. Thirteen polymorphic microsatellite loci were isolated and characterized in 20 to 25 individuals from Manaus (AM, Brazil). The number of alleles per locus varied from 3 to 10 (mean = 6.0). The observed heterozygosity ranged from 0.250 to 0.875 (mean = 0.680) and expected heterozygosity ranged from 0.376 to 0.844 (mean = 0.698). Two loci exhibited null alleles and all loci were in Hardy-Weinberg equilibrium. No linkage disequilibrium between loci was observed.

Conservation genetics of Annamocarya sinensis (Dode) Leroy, an endangered endemic species

Z. Y. Zhang, Pang, X. M., Han, J. W., Wang, Y., and Li, Y. Y., “Conservation genetics of Annamocarya sinensis (Dode) Leroy, an endangered endemic species”, vol. 12, pp. 3965-3974, 2013.

The endangered perennial plant Annamocarya sinensis (Dode) Leroy is a tertiary relict tree restricted to southeastern China and northern Vietnam. To explore endangerment mechanisms, develop protection strategies, and guide reintroduction efforts for this species, we investigated genetic diversity and population structure by surveying 70 individuals from three distinct populations using 12 polymorphic microsatellite markers. We found high genetic diversity for A.

Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species

Q. - Q. Yan, Sun, X. - Q., Guo, J. - L., Hang, Y. - Y., and Li, M. - M., “Development of polymorphic microsatellite markers for Dioscorea zingiberensis and cross-amplification in other Dioscorea species”, vol. 12, pp. 3788-3792, 2013.

Dioscorea zingiberensis C.H. Wright (Dioscoreaceae) is an endemic species in central and southwestern China. In order to study the genetic diversity and population structure of this species, 19 novel polymorphic microsatellite loci were developed using a dual-suppression PCR technique. The number of alleles per locus ranged from 3 to 21, with an average of 9.53. All the markers showed high transferability in cross-species amplification in other species of sect. Stenophora.

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