Microsatellite

Selection of high heterozygosity popcorn varieties in Brazil based on SSR markers

I. B. O. Eloi, Mangolin, C. A., Scapim, C. A., Gonçalves, C. S., and Machado, M. F. P. S., “Selection of high heterozygosity popcorn varieties in Brazil based on SSR markers”, vol. 11, pp. 1851-1860, 2012.

We analyzed genetic structure and diversity among eight populations of popcorn, using SSR loci as genetic markers. Our objectives were to select SSR loci that could be used to estimate genetic diversity within popcorn populations, and to analyze the genetic structure of promising populations with high levels of heterozygosity that could be used in breeding programs. Fifty-seven alleles (3.7 alleles per locus) were detected; the highest effective number of alleles (4.21) and the highest gene diversity (0.763) were found for the Umc2226 locus.

Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)

J. Y. Zheng, Wang, H., Chen, X. X., Wang, P., Gao, P., Li, X. N., and Zhu, G. P., “Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)”, vol. 11, pp. 1975-1980, 2012.

Paris polyphylla var. chinensis is a perennial herb with medicinal properties that is widely used in traditional Chinese medicine. However, this plant has been on the edge of extinction during the last few decades because of excessive deforestation based on the intense ethnopharmaceutical interest. We isolated 12 microsatellite loci from a (CT)n-enriched genomic library of P. polyphylla var. chinensis. The polymorphism at each locus was analyzed by screening 30 individuals from a natural population.

Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)

X. J. Chen, Li, Z. B., Chen, L., Cao, Y. Y., and Li, Q. H., “Isolation and characterization of new microsatellite markers in the pen shell Atrina pectinata (Pinnidae)”, vol. 11, pp. 2884-2887, 2012.

The pen shell, Atrina pectinata, is a commercially important bivalve species, widely consumed in the Asian Pacific region. We identified 16 new microsatellite makers for A. pectinata using a modified fast isolation by AFLP of sequences containing repeat protocols; 27 individuals were collected from Xiamen to evaluate the degree of polymorphism. The number of polymorphic alleles per locus ranged from 2 to 11. The observed and expected heterozygosities were 0.050-0.913 and 0.049-0.869, respectively.

Selection pressures have driven population differentiation of domesticated and wild common carp (Cyprinus carpio L.)

L. H. Xu, Wang, C. H., Wang, J., Dong, Z. J., Ma, Y. Q., and Yang, X. X., “Selection pressures have driven population differentiation of domesticated and wild common carp (Cyprinus carpio L.)”, vol. 11, pp. 3222-3235, 2012.

Selection pressures are the principle evolutionary forces for the genetic differentiation of populations. Recent changes in selection pressures on mitochondrial DNA and microsatellite have been described in a wide variety of organisms. The common carp (Cyprinus carpio) has experienced strong selection pressure, in particular artificial selection, during its domestication. However, the contribution and extent of artificial selection in driving genome-wide population differentiation remain unclear.

Isolation and characterization of 24 polymorphic microsatellite loci in Sepioteuthis lessoniana

X. - D. Zheng, Zuo, Z. - R., Su, X. - J., and Li, Q., “Isolation and characterization of 24 polymorphic microsatellite loci in Sepioteuthis lessoniana”, vol. 11, pp. 3961-3965, 2012.

Owing to their codominant, multiallelic, and highly polymorphic nature, microsatellite markers have been used widely in population genetics and biological resource conservation studies. To investigate the genetic structure of Sepioteuthis lessoniana, we developed 24 microsatellite DNA markers and assessed the polymorphism of each locus in a wild S. lessoniana population. The number of alleles per locus ranged from 4 to 26, and the observed and expected heterozygosities varied from 0.188 to 1.000 and 0.392 to 0.959 with an average of 0.675 and 0.852, respectively.

Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

M. L. Oliveira and Duarte, J. M. B., “Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)”, vol. 12, pp. 44-52, 2013.

We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as “fresh” and 36 as “non-fresh”. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples.

Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)

H. Li, Zhou, Z. - S., Guo, J., and Lin, L. H., “Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)”, vol. 11, pp. 4707-4710, 2012.

We isolated and characterizated 12 polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae). The loci were screened in 37 E. velox individuals. The number of alleles ranged from 6 to 16. The observed heterozygosity ranged from 0.432 to 0.919, and the expected heterozygosity ranged from 0.685 to 0.902. These microsatellite markers should prove useful for population genetic studies of E. velox and other Eremias species.

Isolation and characterization of microsatellite loci for the herbaceous tuber crop, Amorphophallus konjac (Araceae)

C. Pan, You, Y. N., Diao, Y., Hu, Z. L., and Chen, J. M., “Isolation and characterization of microsatellite loci for the herbaceous tuber crop, Amorphophallus konjac (Araceae)”, vol. 11, pp. 4617-4621, 2012.

Amorphophallus konjac is an herbaceous tuber crop with tremendous potential for commercial development. We report the development of microsatellite primers for this important crop species. Thirteen polymorphic microsatellite markers were developed and tested in two populations of A. konjac from the Wuling Mountain Region (WL population) and the Yunnan Province (YN population) in China. The number of alleles per locus ranged from 1 to 7; the observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.844, respectively, in the two populations.

Characterization of eight novel microsatellite markers in the green-lipped mussel Perna viridis (Mytilidae)

Y. Y. Cao, Li, Z. B., Li, Q. H., Chen, X. J., Chen, L., and Dai, G., “Characterization of eight novel microsatellite markers in the green-lipped mussel Perna viridis (Mytilidae)”, vol. 12. pp. 344-347, 2013.

The green lipped mussel, also known as the Asian green mussel (Perna viridis) is a fast reproducing and valuable food source, but it is also considered an invasive species and can clog and damage pipes and marine equipment. Eight novel polymorphic microsatellite loci for P. viridis were isolated and characterized. Microsatellite polymorphism was evaluated in 30 individuals collected from Xiamen, China. The number of alleles per locus and the polymorphism information content ranged from 2 to 5 and from 0.3092 to 0.7031, respectively.

Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers

Y. J. Wang, Zeng, Q. G., and Xu, L. N., “Population structure of the blood clam (Tegillarca granosa) in China based on microsatellite markers”, vol. 12, pp. 892-900, 2013.

The blood clam, Tegillarca granosa, is widely cultivated in China. We isolated 6 microsatellite loci from T. granosa and used them to investigate genetic diversity and population structure of 5 widely distributed populations of blood clam collected from eastern and southeastern China. The allele number per locus varied from 4 to 9, and the polymorphism information content value was 0.301 to 0.830.

Pages

Subscribe to Microsatellite