Microsatellite

Transferability of microsatellites for studies on the social behavior of the tufted capuchin monkey (genus Sapajus)

M. Tokuda, Martins, M. M., and Izar, P., “Transferability of microsatellites for studies on the social behavior of the tufted capuchin monkey (genus Sapajus)”, vol. 13, pp. 9910-9914, 2014.

Because of relevant results that indicated that molecular techniques can provide increased knowledge of animal social systems, they usually complement observational field studies. Despite the great utility of microsatellites, they are not available for all species. Gathering genetic information using microsatellites that were originally designed for other species is a time-saving procedure. The aim of this study was to test the transferability of microsatellites and their usefulness in studies of social behavior of black capuchin monkeys (Sapajus nigritus).

Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization

C. A. Polido, Mantello, C. C., Moraes, A. P., Souza, A. P., and Forni-Martins, E. R., “Microsatellite in Aeschynomene falcata (Leguminosae): diversity, cross-amplification, and chromosome localization”, vol. 13, pp. 10390-10397, 2014.

Aeschynomene falcata is an important forage species; however, because of low seed production, it is underutilized as forage species. Aeschynomene is a polyphyletic genus with a challenging taxonomic position. Two subgenera have been proposed, and it is suggested that Aeschynomene can be split in 2 genera. Thus, new markers, such as microsatellite sequences, are desirable for improving breeding programs for A. falcata.

Use of differential levels of mean observed heterozygosity in microsatellite loci of commercial varieties of sugarcane (Saccharum spp)

R. C. Maranho, Augusto, R., Mangolin, C. A., and Machado, M. F. P. S., “Use of differential levels of mean observed heterozygosity in microsatellite loci of commercial varieties of sugarcane (Saccharum spp)”, vol. 13, pp. 10130-10141, 2014.

In this study, we measured the genetic diversity within and among a set of 9 commercial sugarcane varieties used for alcohol and sugar production using 17 microsatellite DNA markers. The UGSM148 and UGSM59 primers were monomorphic for all 74 sugarcane samples. The estimated proportion of simple sequence repeated (SSR) polymorphic loci was 88.23%; 17 alleles were detected. The mean gene diversity of all SSR loci was 0.7279.

New loci of Lychnophora ericoides and transferability to Lychnophora pinaster, endangered medicinal species from Brazil

M. A. R. Vieira, Marques, M. O. M., Haber, L. L., Vigna, B. B. Z., Bajay, M. M., Pinheiro, J. B., Souza, A. P., Semir, J., and Zucchi, M. I., “New loci of Lychnophora ericoides and transferability to Lychnophora pinaster, endangered medicinal species from Brazil”, vol. 13. pp. 10878-10882, 2014.

Lychnophora ericoides and Lychnophora pinaster are species used in popular medicine as analgesic or anti-inflammatory agents to treat contusions, rheumatism, and insect bites. In this study, 21 simple sequence repeat loci of L. ericoides were developed and transferred to L. pinaster. Three populations of L. ericoides and 2 populations of L. pinaster were evaluated; they were collected in the State of Minas Gerais.

Genetic diversity and population genetic structure of the miiuy croaker, Miichthys miiuy, in the East China Sea by microsatellite markers

Y. Qin, Sun, D. Q., Xu, T. J., Liu, X. Z., and Sun, Y. N., “Genetic diversity and population genetic structure of the miiuy croaker, Miichthys miiuy, in the East China Sea by microsatellite markers”, vol. 13, pp. 10600-10606, 2014.

Genetic diversity and patterns of population structure of the miiuy croaker were investigated using SSR markers. A set of 10 microsatellite loci revealed 40 alleles; the number of alleles varied from 2 to 10 for each marker. A relatively high level of genetic variability was observed between miiuy croaker individuals. Genetic diversity was relatively high within populations with corresponding high average gene flow. There were genealogical branches or clusters corresponding to sampling localities according to the UPGMA tree and principal component analysis.

Isolation and characterization of polymorphic microsatellite loci from the invasive plant Solidago canadensis (Asteraceae)

S. - Y. Zhao, Sun, S. - G., Guo, Y. - H., Chen, J. - M., and Wang, Q. - F., “Isolation and characterization of polymorphic microsatellite loci from the invasive plant Solidago canadensis (Asteraceae)”, vol. 11, pp. 421-424, 2012.

Solidago canadensis, a clonal herb originally from North America (common name: Canada goldenrod), is an invasive species in many countries. We developed microsatellite primers for this species. Eleven polymorphic loci were generated and primers were designed. Polymorphism of these 11 loci was assessed in 35 plants from two populations (Wuhan and Shanghai) in China. The number of alleles per locus ranged from 3 to 14. The observed and expected heterozygosities varied from 0.0732 to 0.7391 and from 0.1177 to 0.8687, respectively.

Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)

W. Peng, Bao, Z. M., Du, H. X., Yan, J. J., Zhang, L. L., and Hu, J. J., “Development and characterization of 70 novel microsatellite markers for the sea cucumber (Apostichopus japonicus)”, vol. 11, pp. 434-439, 2012.

The sea cucumber (Apostichopus japonicus) is an important item in Asian cuisine. It is currently produced through aquaculture, especially in China, after being overexploited in the wild in the 1990s. We isolated 70 novel polymorphic microsatellite loci using an enrichment-colony hybridization protocol. All loci were characterized in 48 individuals from a natural population in Rongcheng (Shandong, China) using genomic DNA isolated from muscle tissue.

Characterization of nine novel microsatellite loci for the Venus clam (Cyclina sinensis)

J. X. Wang, Xu, T. J., Wei, L., Meng, F. X., and Wang, R. X., “Characterization of nine novel microsatellite loci for the Venus clam (Cyclina sinensis)”, vol. 11. pp. 379-382, 2012.

The Venus clam, Cyclina sinensis, is one of the most important bivalves in China marine aquaculture. Using (CA)15-enriched genomic libraries of this species, nine novel polymorphic microsatellite loci were isolated and characterized. The mean number of observed alleles per locus was 16 (range 8-24). The observed and expected heterozygosity ranged from 0.119 to 0.872 and from 0.626 to 0.931, respectively. Three loci had significant departure from Hardy-Weinberg equilibrium and non-significant linkage disequilibrium was found among all nine loci.

Rapid isolation and characterization of polymorphic microsatellite loci in the mud crab, Scylla paramamosain (Portunidae)

H. F. Yao, Sun, D. Q., Wang, R. X., and Shi, G., “Rapid isolation and characterization of polymorphic microsatellite loci in the mud crab, Scylla paramamosain (Portunidae)”, vol. 11, pp. 1503-1506, 2012.

Scylla paramamosain is a widespread and commercially important species of coastal marine crab. We identified 13 polymorphic microsatellite loci from a genome library constructed with 5ꞌ-anchored PCR method. Thirty-two S. paramamosain from the East China Sea were used to analyze the characteristics of these loci. The number of alleles per locus ranged from 3 to 8, with a mean of 5.923. Observed and expected heterozygosities ranged from 0.500 to 0.875 and from 0.500 to 0.859, respectively.

Development and characterization of microsatellite markers for the endangered Chinese yew Taxus chinensis var. mairei (Taxaceae)

H. Xue, Lu, C. H., and Wu, X. B., “Development and characterization of microsatellite markers for the endangered Chinese yew Taxus chinensis var. mairei (Taxaceae)”, vol. 11. pp. 1296-1299, 2012.

We isolated and characterized 11 microsatellite loci for the Chinese yew, Taxus chinensis var. mairei, an endangered tree species in China, by constructing a (CA)12-enriched library. The number of alleles per locus ranged from 5 to 10. The observed heterozygosities ranged from 0.2500 to 0.8333 and the expected heterozygosities ranged from 0.5196 to 0.8680. No significant linkage disequilibrium was detected at these loci. However, four loci significantly deviated from Hardy-Weinberg equilibrium.

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