Genotype

Rifampicin resistance among multi-resistant MRSA clinical isolates from Chennai, India, and their molecular characterization

K. Murugan, Kavitha, K., and Al-Sohaibani, S., Rifampicin resistance among multi-resistant MRSA clinical isolates from Chennai, India, and their molecular characterization, vol. 14, pp. 2716-2725, 2015.

High-level methicillin-resistant Staphylococcus aureus (MRSA) isolates show rapid evolution of rifampicin resistance, forcing reliance upon expensive and often inferior antibiotics to manage these infections. Accordingly, this study was conducted to: 1) evaluate the level of multidrug resistance among hospital-associated MRSA isolates from Chennai, India; 2) determine their rifampicin resistance and molecular characterization; and 3) analyze the identified rpoB gene mutations for predominant high-level rifampicin resistance-associated nucleotide changes.

Genetic polymorphism at the KIR gene locus: determination of gene, genotype, and haplotype frequencies in the Xinjiang Han population

G. - Y. Lin, Yu, B., Hu, W. - J., Zhang, Y. - Z., Zuo, X. - J., and Wang, Y. - B., Genetic polymorphism at the KIR gene locus: determination of gene, genotype, and haplotype frequencies in the Xinjiang Han population, vol. 14, pp. 6213-6222, 2015.

The aim of this study was to explore the genetic polymorphism, genotype, and haplotype characteristics of the KIR locus in the Xinjiang Han population in order to establish a foundation for future analysis of the relationship between KIR genes and disease. KIR genes were detected by sequence-specific primer-polymerase chain reaction in 184 randomly selected, healthy individuals from the Han population in Xinjiang, China. Standard genotype and haplotype analyses were conducted using Hsu’s standards classified for analysis.

Polymorphisms of PRLR and FOLR1 genes and association with milk production traits in goats

J. X. Hou, Fang, F., An, X. P., Yan, Y., Ma, T., Han, P., Meng, F. X., Song, Y. X., Wang, J. G., and Cao, B. Y., Polymorphisms of PRLR and FOLR1 genes and association with milk production traits in goats, vol. 13, pp. 2555-2562, 2014.

We investigated the polymorphisms of PRLR and FOLR1 genes in Xinong Saanen, Guanzhong, and Boer goat breeds by DNA sequencing and PCR–RFLP. Two novel SNPs were identified: KC109741: g.62130C>T in the 3ꞌ-UTR of goat gene PRLR, and KC136296: g.7884A>C in exon 3 of goat gene FOLR1. In the three goat breeds, the polymorphism information content was 0.20-0.27 at the g.62130C>T locus. At the g.7884A>C locus, it was 0.36 in Boer goats.

Phenotypic and molecular characterization of CTX-M-14 extended-spectrum β-lactamase and plasmid-mediated ACT-like AmpC β-lactamase produced by Klebsiella pneumoniae isolates from chickens in Henan Province, China

H. Wu, Liu, B. - G., Liu, J. - H., Pan, Y. - S., Yuan, L., and Hu, G. - Z., Phenotypic and molecular characterization of CTX-M-14 extended-spectrum β-lactamase and plasmid-mediated ACT-like AmpC β-lactamase produced by Klebsiella pneumoniae isolates from chickens in Henan Province, China, vol. 11, pp. 3357-3364, 2012.

Extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases produced by a clinical isolate of Klebsiella pneumoniae from chickens were detected with confirmatory phenotypic tests of the Clinical and Laboratory Standards Institute. The minimum inhibitory concentrations of 18 antibacterial drugs against K. pneumoniae were determined by the 2-fold microdilution method. The genotype and subtype of the ESBL-producing and AmpC β-lactamase-producing K.

Variations in genotype-phenotype correlations in phenylketonuria patients

L. L. Santos, Fonseca, C. G., Starling, A. L. P., Januário, J. N., Aguiar, M. J. B., Peixoto, M. G. C. D., and Carvalho, M. R. S., Variations in genotype-phenotype correlations in phenylketonuria patients, vol. 9, pp. 1-8, 2010.

Phenylalanine hydroxylase deficiency is a trait inherited in an autosomal recessive pattern; the associated phenotype varies considerably. This variation is mainly due to the considerable allelic heterogeneity in the phenylalanine hydroxylase enzyme locus. We examined the genotype-phenotype correlation in 54 phenylketonuria (PKU) patients from Minas Gerais, Brazil. Two systems were used. The first was a phenotype prediction system based on arbitrary values (AV) attributed to each mutation and the second was a correlation analysis.

RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas

J. C. V. Guerra, Jr., Issa, M. R. C., Carneiro, F. E., Strapazzon, R., and Moretto, G., RAPD identification of Varroa destructor genotypes in Brazil and other regions of the Americas, vol. 9, pp. 303-308, 2010.

The mite Varroa destructor is the main pest causing damage to apiculture worldwide. In Brazil and other parts of the world, where bees of African origin and their hybrids predominate, the bees can survive these mites without treatment. Studies have shown a correlation between the various genotypes of the mite and its fertility in different geographical regions. Information about mite genotype could be helpful in understanding the diverse effects and relationships of the mite with bees in different regions of the world.

Molecular epidemiology of the hepatitis C virus in Brazil

S. Busek and Oliveira, G., Molecular epidemiology of the hepatitis C virus in Brazil, vol. 2, pp. 117-123, 2003.

Hepatitis C virus (HCV) is a major cause of liver disease throughout the world. The genome of this virus consists of approximately 10,000 bp and codes for 10 mature polypeptides. Genome sequence comparison has revealed the existence of six major genotypes and a large number of subtypes. The genotypes can be distinguished by whole genome or genome fragment sequencing, geno-type specific amplification of a genomic region or PCR amplification, followed by hybridization or restriction digestion, among other methods.

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