Genetic diversity

Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture

M. Aslam, Awan, F. S., Khan, I. A., and Khan, A. I., Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture, vol. 8, pp. 1459-1465, 2009.

Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were used to test genetic diversity, of which 14 were observed to be polymorphic. Ninety-three loci were amplified; among these, 77 showed polymorphism and the other 16 were monomorphic.

Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified polymorphic DNA

F. A. Khan, Khan, A., Azhar, F. M., and Rauf, S., Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified polymorphic DNA, vol. 8, pp. 1376-1382, 2009.

Genetic diversity of 20 sugarcane accessions in Pakistan was studied using 21 random amplified polymorphic DNA markers. The mean genetic distance between the cultivars was 39.03%, demonstrating that a large part of the genome is similar among the accessions. This probably arises from a lack of parental diversity, with few clones, which are themselves related, contributing to the parentage of these varieties. Among the varieties, none was found to be totally distinct and divergent from the others.

An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers

Y. Erturk, Ercisli, S., Maghradze, D., Orhan, E., and Agar, G., An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers, vol. 8, pp. 1238-1244, 2009.

Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to RAPD (random amplified polymorphic DNA) analysis. We tested 51 random decamer primers; 15 of them gave reproducible polymorphic patterns. These 15 primers produced 226 bands, of which 65% were polymorphic.

RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey

M. Akbulut, Ercisli, S., and Karlidag, H., RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey, vol. 8, pp. 1109-1115, 2009.

The fig tree (Ficus carica L.) is of significant socio-economic importance in Turkey, with 25% of the world’s fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scored after amplification of DNA samples with 13 random primers; 70% of the scored bands were polymorphic. Genetic distances between the fig genotypes ranged from 0.21 to 0.62.

Phenotypic and RAPD diversity among 80 germplasm accessions of the medicinal plant isabgol (Plantago ovata, Plantaginaceae)

N. Singh, Lal, R. K., and Shasany, A. K., Phenotypic and RAPD diversity among 80 germplasm accessions of the medicinal plant isabgol (Plantago ovata, Plantaginaceae), vol. 8, pp. 1273-1284, 2009.

Plantago ovata, popularly known as isabgol, has great commercial and medicinal importance due to thin rosy white membranous seed husk. Isabgol seeds and husks have emollient, demulcent and laxative properties. We used both biometric and molecular techniques to assess the genetic variability and relatedness of 80 germplasm accessions of Plantago spp (P. ovata, P. lanceolata, and P. major) collected both from India and abroad. The range of D2 values (2.01-4890.73) indicated a very high degree of divergence among the accessions.

Genetic diversity in wild (Sus scrofa scrofa) and domestic (Sus scrofa domestica) pigs and their hybrids based on polymorphism of a fragment of the D-loop region in the mitochondrial DNA

S. F. Grossi, Lui, J. F., Garcia, J. E., and Meirelles, F. V., Genetic diversity in wild (Sus scrofa scrofa) and domestic (Sus scrofa domestica) pigs and their hybrids based on polymorphism of a fragment of the D-loop region in the mitochondrial DNA, vol. 5, pp. 564-568, 2006.

We examined the variation in mitochondrial DNA by sequencing the D-loop region in wild and domestic (large-white breed) pigs, in hybrids between domestic and wild pigs, and in Monteiro pigs. A D-loop fragment of approximately 330 bp was amplified by PCR. Sequencing of DNA amplicons identified haplotypes previously described as European and Asian types. Monteiro pigs and wild pigs had European haplotypes and domestic pigs had both European and Asian haplotypes.

Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA

A. Iqbal, Khan, A. S., Khan, I. A., Awan, F. S., Ahmad, A., and Khan, A. A., Study of genetic divergence among wheat genotypes through random amplified polymorphic DNA, vol. 6, pp. 476-481, 2007.

The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. Among the 112, 50 fragments showed polymorphism among the seven wheat genotypes. Nei and Li’s similarity matrix ranged from 86.2 to 93.0%, which indicated a narrow genetic base among the genotypes. The maximum similarity, 93.0%, was observed between 12WLRG/1-12 and WL-43.

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