Gene expression

Regulation of WNK4 gene transcription in the kidneys

Z. J. Sun, Li, Y., Li, Y. H., Zhang, J. S., Ding, Q., Li-Ling, J., Liang, Y., and Zhao, Y. Y., Regulation of WNK4 gene transcription in the kidneys, vol. 12, pp. 2332-2340, 2013.

With-no-lysine (K) kinase-4 (WNK4) is a newly cloned kinase-encoding gene that plays a crucial role in the maintenance of electrolyte homeostasis. Mutations of WNK4 can cause pseudohypoal­dosteronism type α, an autosomal dominant disease characterized by hyperkalemia, metabolic acidosis and hypertension. We explored the expression and regulatory mechanism of WNK4 in the human kidneys, which is a key regulator of blood pressure. Expression of WNK4 was determined by RT-PCR.

Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.)

J. Zhang, Sun, X. L., Zhang, L. G., Hui, M. X., and Zhang, M. K., Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.), vol. 12, pp. 2507-2516, 2013.

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain reaction (real-time PCR).

Age-dependent expression of the BPI gene in Sutai piglets

J. Zhu, Zi, C., Wu, Z. C., Liu, L., Zheng, X. R., Su, X. M., Zhu, G. Q., Huang, X. G., Wu, S. L., and Bao, W. B., Age-dependent expression of the BPI gene in Sutai piglets, vol. 12, pp. 2120-2126, 2013.

We compared and analyzed the expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18 and 35-day expres­sion in the jejunum. There were also significant differences between 35-day expression and expression at the other development stages in the duodenum. There were no significant differences in expression at 8, 18, and 30 days in the jejunum.

Gene expression in the lignin biosynthesis pathway during soybean seed development

A. Baldoni, Von Pinho, E. V. R., Fernandes, J. S., Abreu, V. M., and Carvalho, M. L. M., Gene expression in the lignin biosynthesis pathway during soybean seed development, vol. 12, pp. 2618-2624, 2013.

The study of gene expression in plants is fundamental, and understanding the molecular mechanisms involved in important biological processes, such as biochemical pathways or signaling that are used or manipulated in improvement programs, are key for the production of high-quality soybean seeds. Reports related to gene expression of lignin in seeds are scarce in the literature.

Roles of functional NFKB1 and β-TrCP insertion/deletion polymorphisms in mRNA expression and epithelial ovarian cancer susceptibility

Z. H. Huo, Zhong, H. J., Zhu, Y. S., Xing, B., and Tang, H., Roles of functional NFKB1 and β-TrCP insertion/deletion polymorphisms in mRNA expression and epithelial ovarian cancer susceptibility, vol. 12, pp. 3435-3443, 2013.

Epithelial ovarian cancer (EOC) is the leading cause of death among all gynecological cancers. Nuclear factor-kappa B (NF-κB) is involved in carcinogenesis and in the development of EOC. The β-transducin repeat-containing protein (β-TrCP) is a positive regulator of the NF-κB signaling pathway. Recent studies have indicated that the -94 ins/del ATTG polymorphism in the promoter region of the NFKB1 gene, and the 9N ins/del polymorphism in the 3ꞌ-untranslated region of the β-TrCP gene are associated with increased susceptibility to a variety of cancers.

Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases

Y. Q. Liu, Tao, D. C., Liao, S. Y., Yang, Y., Ma, Y. X., and Zhang, S. Z., Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases, vol. 12, pp. 4060-4069, 2013.

Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein 148 (RNF148) that is abundantly expressed in testes and slightly expressed in pancreas.

Expression of cationic amino acid transporters, carcass traits, and performance of growing pigs fed low-protein amino acid-supplemented versus high protein diets

A. Morales, Grageola, F., García, H., Araiza, A., Zijlstra, R. T., and Cervantes, M., Expression of cationic amino acid transporters, carcass traits, and performance of growing pigs fed low-protein amino acid-supplemented versus high protein diets, vol. 12, pp. 4712-4722, 2013.

Free amino acids (AA) appear to be absorbed faster than protein-bound AA (PB-AA). We conducted an experiment to assess the effect of feeding pigs with a partially free (F-AA) or totally PB-AA diet on expression of selected genes and performance of pigs. The expression of cationic AA transporters b0,+ and CAT-1 in intestinal mucosa, liver, and longissimus (LM) and semitendinosus (SM) muscles, as well as that of myosin in LM and SM, was analyzed. Twelve pigs (31.7 ± 2.7 kg) were used.

Aberrant gene expression profiles in hepatocellular carcinoma detected by microdissection

Y. M. Wei, Li, Y. Y., Zhang, Y. C., and Nie, Y. Q., Aberrant gene expression profiles in hepatocellular carcinoma detected by microdissection, vol. 12, pp. 5527-5536, 2013.

The aim of this study was to identify genomic aberrations in hepatocellular carcinoma (HCC) by using laser capture microdissection (LCM) combined with microarray analysis. Samples were procured by LCM from HCC and patient-matched normal liver tissue surgically resected from 4 patients. RNA was isolated from the samples and reverse transcribed into cDNA. After 2-cycle linear amplification and 2-color fluorescent labeling, the cRNA was hybridized onto a whole genome microarray. All genes expressed in the normal and HCC samples were counted and analyzed.

Critical evaluation of transcription factor Atf2 as a candidate modulator of alcohol preference in mouse and human populations

L. S. Wang, Jiao, Y., Huang, Y., Liu, X. Y., Gibson, G., Bennett, B., Hamre, K. M., Li, D. W., Zhao, H. Y., Gelernter, J., Kranzler, H. R., Farrer, L. A., Lu, L., Wang, Y. J., and Gu, W. K., Critical evaluation of transcription factor Atf2 as a candidate modulator of alcohol preference in mouse and human populations, vol. 12, pp. 5992-6005, 2013.

In prior work, congenic strains carrying the DBA/2Igb (D2) region of chromosome 2 (Chr2) for alcohol preference were bred onto a C57BL/6Ibg (B6) background and as predicted were found to reduce voluntary consumption. Subsequently, interval-specific congenic recombinant strains (ISCRS) were generated and also tested. These ISCRS strains reduced the quantitative trait loci (QTL) interval to a comparatively small 3.4 Mb region. Here, we have exploited an integrative approach using both murine and human populations to critically evaluate candidate genes within this region.

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