Gene expression

Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines

J. Ogorevc and Dovč, P., Relative quantification of beta-casein expression in primary goat mammary epithelial cell lines, vol. 14, pp. 3481-3490, 2015.

Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established primary cell lines in the second passage were grown in basal medium on plastic and in hormone-supplemented (lactogenic) medium on plastic and on an extracellular matrix-covered surface, respectively.

Correlation between sheep YAP1 temporal and spatial expression trends and MSTN and MyoG gene expression

X. Y. Lv, Sun, W., Su, R., Li, D., Wang, Q. Z., Musa, H. H., Chen, L., Zhang, Y. F., and Wu, W. Z., Correlation between sheep YAP1 temporal and spatial expression trends and MSTN and MyoG gene expression, vol. 14, pp. 3244-3256, 2015.

The aim of the current study was to investigate the effects of Yes-associated protein 1 (YAP1) gene expression after birth on the development of muscle and the relationship between YAP1 and myostatin (MSTN) and myogenin (MyoG).

Molecular cloning and expression analysis of TRAF3 in chicken

H. L. Yang, Feng, Z. Q., Zeng, S. Q., Li, S. M., Zhu, Q., and Liu, Y. P., Molecular cloning and expression analysis of TRAF3 in chicken, vol. 14, pp. 4408-4419, 2015.

Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (chTRAF3) and detected its tissue expression profile in chicken.

Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

W. Y. Zhang, Jiang, S. F., Xiong, Y. W., Fu, H. T., Qiao, H., Sun, S. M., Gong, Y. S., and Jin, S. B., Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense, vol. 14, pp. 4318-4330, 2015.

The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its expression in crustaceans.

Transcriptome analysis of Arabidopsis seedlings responses to high concentrations of glucose

L. Han, Li, J. L., Jin, M., and Su, Y. H., Transcriptome analysis of Arabidopsis seedlings responses to high concentrations of glucose, vol. 14, pp. 4784-4801, 2015.

Sugars acting as fuel energy or as signaling molecules play important roles in plant growth and development. Although sugars associated with early seedling development have been analyzed in detail, few studies have examined the effect of sugar on genome-wide gene transcription. To analyze the role of glucose on the genomic level, we examined the response of seedlings to 5% glucose using RNA-seq technology. High concentrations of glucose significately altered the expression of 863 genes, with 558 upregulated and 305 downregulated genes by more than 2-fold.

Meta-analysis of differentially expressed genes in ankylosing spondylitis

Y. H. Lee and Song, G. G., Meta-analysis of differentially expressed genes in ankylosing spondylitis, vol. 14, pp. 5161-5170, 2015.

The purpose of this study was to identify differentially expressed (DE) genes and biological processes associated with changes in gene expression in ankylosing spondylitis (AS). We performed a meta-analysis using the integrative meta-analysis of expression data program on publicly available microarray AS Gene Expression Omnibus (GEO) datasets. We performed Gene Ontology (GO) enrichment analyses and pathway analysis using the Kyoto Encyclopedia of Genes and Genomes. Four GEO datasets, including 31 patients with AS and 39 controls, were available for the meta-analysis.

Study of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana

H. X. Zhao, Zeng, X. N., Liang, Q., Zhang, X. F., Huang, W. Z., Chen, H. S., and Luo, Y. X., Study of the obp5 gene in Apis mellifera ligustica and Apis cerana cerana, vol. 14, pp. 6482-6494, 2015.

Apis mellifera ligustica and A. cerana cerana exhibit differences in olfactory sensitivity to odors from nectariferous plants and diseased broods. It is presumed that the differences in odorant-binding proteins (OBPs) between these 2 species contribute to their olfactory sensitivity. We compared the sequences, temporal expression pattern, and binding properties of the 2 OBP-encoding genes. We cloned the Amobp5 and Acobp5 genes.

Partial least squares-based gene expression analysis in preeclampsia

F. Jiang, Yang, Y., Li, J., Li, W., Luo, Y., Li, Y., Zhao, H., Wang, X., Yin, G., and Wu, G., Partial least squares-based gene expression analysis in preeclampsia, vol. 14, pp. 6598-6604, 2015.

Preeclampsia is major cause of maternal and fetal morbidity and mortality. Currently, the etiology of preeclampsia is unclear. In this study, we investigated differences in gene expression between preeclampsia patients and controls using partial least squares-based analysis, which is more suitable than routine analysis. Expression profile data were downloaded from the Gene Expression Omnibus database. A total of 503 genes were found to be differentially expressed, including 248 downregulated genes and 255 overexpressed genes.

Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.)

H. Q. Han, Liu, Y., Jiang, M. M., Ge, H. Y., and Chen, H. Y., Identification and expression analysis of YABBY family genes associated with fruit shape in tomato (Solanum lycopersicum L.), vol. 14, pp. 7079-7091, 2015.

YABBY family genes play important roles in the development of leaf, flower, and fruit. The purpose of this research was to integrate all the YABBY genes and analyze the correlation between gene expression and fruit shape in tomato. Scanning of 24 genomes of sequenced species demonstrated that YABBY genes were very normal and stable in flowering plants except the seedless plants. Nine YABBY genes in tomato were computationally and experimentally characterized.

Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 in pig adipose tissue

R. L. Qi, Chen, Y., Huang, J. X., and Yang, F. Y., Effects of conjugated linoleic acid on the expression levels of miR-27 and miR-143 in pig adipose tissue, vol. 14, pp. 6985-6992, 2015.

In this study, we evaluated the effect and possible mech­anism of action of dietary conjugated linoleic acid (CLA) on pig body fat deposition. Landrace piglets (N = 48) were randomly divided into three groups, which were fed diets containing 0% (control), 1%, or 2% CLA. Dorsal and abdominal subcutaneous adipose tissues were col­lected, and real-time polymerase chain reaction (PCR) was used to de­termine the expression of adipocyte differentiation marker genes and associated microRNAs (miRNAs).

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