Differential expression

Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat

Z. Q. Ren, Wu, W. J., Liu, W. H., Zheng, R., Li, J. L., Zuo, B., Xu, D. Q., Li, F. E., Lei, M. G., Ni, D. B., and Xiong, Y. Z., Differential expression and effect of the porcine ANGPTL4 gene on intramuscular fat, vol. 13, pp. 2949-2958, 2014.

In a previous study, we investigated differences in gene expression in backfat between Meishan and Large White pigs and their F1 hybrids, Large White x Meishan, and Meishan x Large White pigs. One potential differentially expressed sequence tag from the mRNA differential display was a homolog of the human angiopoietin-like 4 (ANGPTL4) gene, which encodes a protein that is secreted by both liver and white adipose tissues and can inhibit lipoprotein lipase activity and stimulate white adipose tissue lipolysis.

MicroRNA network analysis identifies key microRNAs and genes associated with precancerous lesions of gastric cancer

X. W. Wang, Wu, Y., Wang, D., and Qin, Z. F., MicroRNA network analysis identifies key microRNAs and genes associated with precancerous lesions of gastric cancer, vol. 13, pp. 8695-8703, 2014.

To identify potential targets for the early treatment and prevention of gastric cancer, microRNA (miRNA) expression profiles of precancerous lesions of gastric cancer were investigated. The miRNA microarray dataset GSE24839 was downloaded from Gene Expression Omnibus (GEO) and included 10 Helicobacter pylori-related gastritis samples and 10 gastric intestinal metaplasia samples. Differentially expressed miRNAs (DEMs) were screened using the Student t-test; P H.

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

S. J. Liu, Chen, F. F., Wu, C., Ni, Q. S., and Yu, W. Y., Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata), vol. 13, pp. 8971-8981, 2014.

Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for MDIi-2 from healthy adult Muscovy Duck. The full-length MDIi-2 cDNA sequence was found to be 1377-base pairs, encoding a 285-amino acid protein.

Cassava (Manihot esculenta Krantz) genome harbors KNOX genes differentially expressed during storage root development

D. Guo, Li, H. L., Tang, X., and Peng, S. Q., Cassava (Manihot esculenta Krantz) genome harbors KNOX genes differentially expressed during storage root development, vol. 13, pp. 10714-10726, 2014.

In plants, homeodomain proteins play a critical role in regulating various aspects of plant growth and development. KNOX proteins are members of the homeodomain protein family. The KNOX transcription factors have been reported from Arabidopsis, rice, and other higher plants. The recent publication of the draft genome sequence of cassava (Manihot esculenta Krantz) has allowed a genome-wide search for M. esculenta KNOX (MeKNOX) transcription factors and the comparison of these positively identified proteins with their homologs in model plants.

Analysis of differentially expressed genes in various stages of Duchenne muscular dystrophy by using a network view

D. Wang, Wang, Z. J., Song, X. X., Pu, L. H., Li, X., and Wang, Y., Analysis of differentially expressed genes in various stages of Duchenne muscular dystrophy by using a network view, vol. 12, pp. 4480-4488, 2013.

Duchenne muscular dystrophy (DMD), which is caused by mutations in the X-linked dystrophin gene, is a severe and progressive neuromuscular disease with no available cure. By integrating 2 microarray datasets from the Gene Expression Omnibus, we identified differentially expressed genes in 2 stages of DMD and systematically explored their potential disease-related mechanisms using a network view. Twenty differentially expressed genes were detected in various stages of DMD.

Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata)

S. J. Liu, Chen, F. F., Dai, Y., Wu, C., Ni, Q. S., and Yu, W. Y., Molecular characterization and tissue-specific expression of invariant chain in the muscovy duck (Cairina moschata), vol. 10, pp. 2867-2880, 2011.

The invariant chain (Ii) plays an important role as a chaperone for MHC II maturation and facilitates antigen presentation in vertebrates. We cloned, characterized and made a homology analysis of healthy adult muscovy duck Ii (MDIi), from a poultry farm in the suburban district of Hefei city in China, by rapid amplification of cDNA ends (RACE)-PCR and by measuring expression of the MDIi gene in various tissues by real-time quantitative PCR. A full-length cDNA sequence of MDIi was obtained, 1188-bp long, encoding a 222-amino acid protein.

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