Diagnosis

Accuracy of enzyme-linked immunospot assay for diagnosis of pleural tuberculosis: a meta-analysis

Z. Z. Li, Qin, W. Z., Li, L., Wu, Q., and Wang, Y. J., Accuracy of enzyme-linked immunospot assay for diagnosis of pleural tuberculosis: a meta-analysis, vol. 14, pp. 11672-11680, 2015.

Current methods for diagnosing tuberculous pleurisy are poor. Some studies have explored the diagnostic value of a pleural effusion enzyme-linked immunospot (ELISPOT) assay, but its accuracy remains controversial. Therefore, we performed a meta-analysis of the existing evidence on the ability of the ELISPOT assay to diagnose tuberculous pleurisy. We systematically searched PubMed, Google Scholar, and EMBASE databases for studies measuring the sensitivity, specificity, and other measures of accuracy of the pleural effusion ELISPOT assay for diagnosis of tuberculous pleurisy.

Clinical applications of internal heat source analysis for breast cancer identification

F. Han, Liang, C. W., Shi, G. L., Wang, L., and Li, K. Y., Clinical applications of internal heat source analysis for breast cancer identification, vol. 14, pp. 1450-1460, 2015.

Nondestructive preoperative breast imaging techniques are widely used for breast cancer testing and diagnosis. This study aimed to evaluate the feasibility and efficacy of quantitative diagnosis via the thermal analysis of abnormal metabolism. Nine hundred forty-eight women who underwent breast biopsy from 2009 to 2013 were investigated. Thermal analysis was used to calculate the internal heat source (i.e., tumor) thermal power for each participant.

Muscle pain, fever, cough, and progressive dyspnea in a woman with eosinophilic pneumonia

Z. Xu, Fan, Y., Wang, G. S., and Wu, G. M., Muscle pain, fever, cough, and progressive dyspnea in a woman with eosinophilic pneumonia, vol. 14. pp. 4189-4194, 2015.

The diagnosis of eosinophilic pneumonia (EP) is rare and challenging. This condition is frequently misdiagnosed as pulmonary tuberculosis, lymphoma, schistosomiasis, Wegener’s granuloma, severe acute respiratory syndrome, or severe community-acquired pneumonia. Herein, we report a case in which computed tomography (CT)-guided percutaneous lung biopsy was used to diagnose EP without alveolar eosinophilia or peripheral eosinophilia. A roundworm identified in the patient’s stool confirmed the precise diagnosis to be parasitic EP.

Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil

R. P. Araldi, Carvalho, R. F., Melo, T. C., Diniz, N. S. P., Sant’Ana, T. A., Mazzuchelli-de-Souza, J., Spadacci-Morena, D. D., Beçak, W., and Stocco, R. C., Bovine papillomavirus in beef cattle: first description of BPV-12 and putative type BAPV8 in Brazil, vol. 13, pp. 5644-5653, 2014.

Bovine papillomavirus (BPV) is an oncogenic virus associated with benign and malignant lesions, which result in notable economic losses. Peripheral blood samples and cutaneous papillomas were obtained from four adult beef cattle. Viral molecular identification was performed using specific primers for BPV-1, -2 and -4 in blood diagnosis and FAP59/FAP64 for skin papillomas. Histopathologic examination was done as a complementary and differential diagnosis. The fragments were purified, sequenced, and compared using BLASTn.

Clinical significance of neutrophil gelatinase-associated lipocalin (NGAL) in colorectal cancer: a meta-analysis

Y. Wang and Zeng, T. T., Clinical significance of neutrophil gelatinase-associated lipocalin (NGAL) in colorectal cancer: a meta-analysis, vol. 13, pp. 7102-7112, 2014.

Growing evidence has implicated that neutrophil gelatinase-associated lipocalin (NGAL) plays a role in a spectrum of human cancers. Several observational studies from different parts of the world have been devoted to elucidate the clinical relationship between NGAL and colorectal cancer. This meta-analysis aimed to explore the overall accuracy of NGAL detection for the diagnosis and prognosis of colorectal cancer. Based on comprehensive literature screening on Pubmed, Ovid, and CNKI databases, our screening covered all published papers until March 2013.

Selection of peptides for serological detection of equine infectious anemia

E. M. Santos, Cardoso, R., Souza, G. R. L., Goulart, L. R., Heinemann, M. B., Leite, R. C., and Reis, J. K. P., Selection of peptides for serological detection of equine infectious anemia, vol. 11, pp. 2182-2199, 2012.

Equine infectious anemia caused by equine infectious anemia virus is an important disease due to its high severity and incidence in animals. We used a phage display library to isolate peptides that can be considered potential markers for equine infectious anemia diagnosis. We selected peptides using IgG purified from a pool comprised of 20 sera from animals naturally infected with equine infectious anemia virus. The diagnostic potential of these peptides was investigated by ELISA, Western blot and dot blot with purified IgG and serum samples.

Significance of Bcl10 gene mutations in the clinical diagnosis of MALT-type ocular adnexal lymphoma in the Chinese population

J. Zhu, Wei, R. L., Pi, Y. L., and Guo, Q., Significance of Bcl10 gene mutations in the clinical diagnosis of MALT-type ocular adnexal lymphoma in the Chinese population, vol. 12, pp. 1194-1204, 2013.

We investigated the expression of Bcl10 gene mutations in mucosa-associated lymphoid tissue-type ocular adnexal lymphoma (OAL), atypical lymphoid hyperplasia (ALH), and reactive lymphoid hyperplasia (RLH) in the Chinese population and its role in clinical diagnosis and pathogenesis. Forty-three samples were collected during patient surgeries. Pathological diagnosis confirmed OAL in 23 cases, ALH in 10 cases, and RLH in 10 cases. Normal peripheral lymph tissues from 12 cases were used as negative controls.

Clinical value of surfactant protein-A in serum and sputum for pulmonary tuberculosis diagnosis

H. Hu, Teng, G. L., Gai, L. Z., Yang, Y., and Zhu, C. J., Clinical value of surfactant protein-A in serum and sputum for pulmonary tuberculosis diagnosis, vol. 12, pp. 4918-4924, 2013.

The aim of this study was to explore the diagnostic and differential diagnosis value of surfactant protein-A (SP-A) in the serum and sputum for pulmonary tuberculosis. A total of 101 patients with pulmonary tuberculosis, 85 healthy volunteers, and 30 chronic obstructive pulmonary disease (COPD) patients were divided into pulmonary tuberculosis group, healthy control group, and COPD group, respectively. SP-A was determined in the serum and sputum in the three groups by enzyme-linked immunosorbent assay. The expression of SP-A in serum was significantly higher (P 0.05).

Combined detection of p53, p16, Rb, and EGFR mutations in lung cancer by suspension microarray

Y. Ye, Wang, D., Su, C., Rong, T., and Guo, A., Combined detection of p53, p16, Rb, and EGFR mutations in lung cancer by suspension microarray, vol. 8, pp. 1509-1518, 2009.

Mutations of some contributing factors (p53, p16, Rb, and EGFR) are believed to affect diagnosis and drug resistance of lung cancer. We evaluated the efficacy of a multimarker panel for molecular diagnosis of lung cancer, using a high-throughput suspension microarray. One hundred and twenty-five lung cancer specimens and 30 tumor-free lung tissue samples were assayed by multiplex polymerase chain reaction with specific probes designed to detect hot-spot mutations in p53, p16, Rb, and EGFR.

Diagnosis of human visceral leishmaniasis by PCR using blood samples spotted on filter paper

E. Sergio da Silva, Gontijo, C. Maria Ferr, Pacheco, Rda Silva, and Brazil, R. Peçanha, Diagnosis of human visceral leishmaniasis by PCR using blood samples spotted on filter paper, vol. 3, pp. 251-257, 2004.

The polymerase chain reaction (PCR) is a simple, rapid procedure that has been adapted for the diagnosis of leishmaniasis. In the present study, 85 blood samples and seven bone marrow aspirates from 85 patients with clinical symptoms suggestive of visceral leishmaniasis from the metropolitan region of Belo Horizonte in the Brazilian State of Minas Gerais were screened using molecular and serological techniques. Samples that were negative (N = 12) and positive (N = 19) in parasitological and serological tests were used as controls.

Subscribe to Diagnosis