Cross-species amplification

Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification

H. B. Liu, Liu, S. F., Ye, J. B., Yuan, Y. J., Ding, S. X., and Zhuang, Z. M., “Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification”, vol. 13. pp. 134-138, 2014.

A (GT/CA)13-microsatellite-enriched genomic library of the false kelpfish Sebastiscus marmoratus was constructed, and 20 polymorphic microsatellite loci were isolated and characterized. The polymorphisms were investigated in 48 wild individuals from a single population collected from the northern Yellow Sea. The numbers of alleles per locus varied from 4-22 with an average of 9. The observed and expected heterozygosities of each locus ranged from 0.196-0.958 and from 0.487-0.942, with an average of 0.693 and 0.765, respectively.

Assessment of microsatellites in estimating inter- and intraspecific variation among Neotropical Crocodylus species

A. Bashyal, Gross, B. A., Venegas-Anaya, M., Lowrance, F., and Densmore, III, L. D., “Assessment of microsatellites in estimating inter- and intraspecific variation among Neotropical Crocodylus species”, vol. 13, pp. 5492-5502, 2014.

We tested microsatellites that were developed for the saltwater crocodile (Crocodylus porosus) for cross-species amplification and to provide an estimate of inter- and intraspecific variation among four species of Neotropical crocodiles (C. rhombifer, C. intermedius, C. acutus, and C. moreletii). Our results indicated that with the exception of 2 loci in C.

Development of microsatellite markers for the small yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species amplification

D. Q. Sun, Li, H. Y., Xu, T. J., and Wang, R. X., “Development of microsatellite markers for the small yellow croaker Larimichthys polyactis (Sciaenidae) by cross-species amplification”, vol. 11, pp. 1469-1474, 2012.

The small yellow croaker (Larimichthys polyactis) is a highly valued fish for human consumption found in the Western Pacific that was considered endangered until recently because of overfishing. We selected microsatellite markers for this species from markers developed for Miichthys miiuy, also of the family Sciaenidae. Among 43 markers polymorphic for M. miiuy, 11 were found to be polymorphic for L. polyactis. Characterization of these 11 loci was made based on 30 L.

Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations

D. Kaczmarczyk, “Cross-species amplification of selected zebrafish, central stoneroller, and finescale dace microsatellites in lake minnow populations”, vol. 12. pp. 154-159, 2013.

Fifteen sets of PCR primers designed for the amplification of microsatellite loci from Danio rerio and Phoxinus neogaeus Campostoma DNA that have been proven applicable for molecular studies of several species of cyprinids were tested for amplification of microsatellites from lake minnow DNA. The samples were taken from 298 fish inhabiting 6 distinct populations located in Poland, and DNA was extracted from dried fin clips.

Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus

P. - Z. Qi, Xie, C. - X., Guo, B. - Y., Wu, C. - W., Lu, S. - M., and Duan, Y. - J., “Development of new polymorphic microsatellite markers in topmouth culter (Culter alburnus) and determination of their applicability in Culter mongolicus”, vol. 12, pp. 1761-1765, 2013.

Fifteen new polymorphic microsatellite markers were developed for Culter alburnus. In 32 individuals representing a wild population of the Danjiangkou Reservoir, Hubei, China, the number of alleles at these loci varied between 2 and 10, with an average of 5.5. The average observed and expected heterozygosities were 0.664 and 0.681, respectively. The polymorphism information content of 11 loci was more than 0.5 whereas that of the other 4 loci was less than 0.5 but more than 0.25. In addition, the genomes of 30 C.

Characterization and cross-species amplification of microsatellite markers in African Silverbill (Lonchura cantans)

N. R. Parine, Kumar, D., Pathan, A. A. K., Elrobh, M. S., Khan, W., and Alanazi, M., “Characterization and cross-species amplification of microsatellite markers in African Silverbill (Lonchura cantans)”, vol. 12, pp. 5634-5639, 2013.

We tested the cross-amplification of eight microsatellites developed for Bengalese finch in African Silverbill (Lonchura cantans). In order to develop resources for conservation genetic studies in the species L. cantans, we tested the amplification success and polymorphism in eight previously developed microsatellite loci, in L. cantans. All eight microsatellite markers were successfully amplified, of which all were polymorphic, with 3 to 9 alleles and an expected heterozygosity (HE) ranging from 0.606 to 0.718.

Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes

Y. - J. Xu, Liao, M. - J., Wang, Y. - G., Liu, Z. - C., Qin, P., Zhang, Z., Rong, X. - J., and Liu, X. - Z., “Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes”, vol. 12, pp. 6767-6772, 2013.

We investigated the genetic diversity of the southern flounder Paralichthys lethostigma. Microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. Cross-species amplification was also conducted in five pleuronectiforme species. Of 45 randomly selected and sequenced clones, 43 contained a CA or GA repeat motif. Fourteen pairs of primers were designed to investigate the polymorphism and genetic structure of a wild population collected from North Carolina State coastal waters.

Transferability of microsatellite markers among economically and ecologically important galliform birds

N. Bech, Novoa, C., Allienne, J. F., and Boissier, J., “Transferability of microsatellite markers among economically and ecologically important galliform birds”, vol. 9, pp. 1121-1129, 2010.

We used the partially sequenced genomes of the turkey and chicken to find a large number of microsatellite markers. We then characterized 10 polymorphic microsatellite markers developed by cross-species amplification from economically and ecologically important birds to various European sub-species of the grey partridge. Even though we used cross-species amplification, a high degree of polymorphism was conserved in all microsatellite markers.

Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)

J. E. Maldonado, Cosse, M., and González, S., “Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)”, vol. 6, pp. 1118-1122, 2007.

In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847.

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