BAC

New genomic resources for the honey bee (Apis mellifera L.): development of a deep-coverage BAC library and a preliminary STC database

J. P. Tomkins, Luo, M., Fang, G. C., Main, D., Goicoechea, J. L., Atkins, M., Frisch, D. A., Page, R. E., Guzmán-Novoa, E., Yu, Y., Hunt, G., and Wing, R. A., New genomic resources for the honey bee (Apis mellifera L.): development of a deep-coverage BAC library and a preliminary STC database, vol. 1, pp. 306-316, 2002.

We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size of 113 kb (range = 27 to 213 kb) and 2% empty vectors. Based on an estimated genome size of 270 Mb, this library provides approximately 15 haploid genome equivalents, allowing >99% probability of recovering any specific sequence of interest.

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