Association

Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus

N. Ghodsian, Akhlaghi, M., Ramachandran, V., Heidari, F., Haghvirdizadeh, P., Eshkoor, S. A., Etemad, A., Jamaluddin, J. A., and Ismail, P., “Association of TNF-α G308A gene polymorphism in essential hypertensive patients without type 2 diabetes mellitus”, vol. 14, pp. 18974-18979, 2015.

This study aims to investigate the effects of tumor necrosis factor alpha (TNF-α) G308A gene polymorphism on essential hypertension (EHT) with or without type 2 diabetes mellitus (T2DM). The project was conducted on buccal epithelial and blood cells for case and control patients, respectively. Epithelial cells were obtained from the inner part of the cheeks. Techniques including DNA extraction, polymerase chain reaction (PCR), and restriction fragment length polymorphism (RFLP) were utilized to assess biomarkers of DNA damage.

Polymorphisms in the bone morphogenetic protein 15 gene and their effect on sperm quality traits in Chinese Holstein bulls

L. P. Sun, Song, Y. P., Du, Q. Z., Song, L. W., Tian, Y. Z., Zhang, S. L., Hua, G. H., and Yang, L. G., “Polymorphisms in the bone morphogenetic protein 15 gene and their effect on sperm quality traits in Chinese Holstein bulls”, vol. 13, pp. 1805-1812, 2014.

Bone morphogenetic protein 15 (BMP-15) expression has been detected in the testis, but its roles in this organ has not been well elucidated. We evaluated polymorphisms of the BMP-15 gene by PCR-SSCP and PCR-RFLP in 212 Chinese Holstein bulls, and investigated possible associations with sperm quality traits, including semen volume per ejaculate, sperm density, fresh sperm motility, thawed sperm motility, acrosome integrity rate, and abnormal sperm rate. A single nucleotide polymorphism (C5697T) in intron 1 of the BMP-15 gene was identified in these bulls.

Angiotensin-converting enzyme insertion/deletion polymorphism and susceptibility to systemic sclerosis: a meta-analysis

G. G. Song and Lee, Y. H., “Angiotensin-converting enzyme insertion/deletion polymorphism and susceptibility to systemic sclerosis: a meta-analysis”, vol. 13, pp. 8174-8183, 2014.

The purpose of this study was to examine whether the insertion (I) or deletion (D) polymorphism of the angiotensin-converting enzyme gene (ACE) is associated with susceptibility to systemic sclerosis (SSc). A meta-analysis examining the associations between the ACE I/D polymorphism and SSc was conducted in overall and European populations using 1) allelic contrast (D vs I); 2) recessive (DD vs ID + II); 3) dominant (DD + ID vs II); and 4) additive (DD vs ID vs II) models.

Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds

J. Wang, Wang, C., Tian, R., Huang, Y. - Z., Lai, X. - S., Lan, X. - Y., Wang, J. - Q., and Chen, H., “Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds”, vol. 11, pp. 746-755, 2012.

As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms in exons 2, 3, 4, 5, 7, 8, and 9 of the PRDM16 gene were detected by PCR-SSCP, DNA sequencing and CRS-PCR-RFLP methods in 1031 cattle of the Chinese breeds: Jiaxian, Nanyang, Qinchuan, and Chinese Holstein.

Agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers

A. Kalivas, Xanthopoulos, F., Kehagia, O., and Tsaftaris, A. S., “Agronomic characterization, genetic diversity and association analysis of cotton cultivars using simple sequence repeat molecular markers”, vol. 10, pp. 208-217, 2011.

Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the Greek market, as Greece is the largest producer in Europe. We used simple sequence repeat (SSR) markers for the identification and the phylogenetic analysis of the most widely cultivated cotton cultivars in Greece.

Back fat thickness and meat tenderness are associated with a 526 T→A mutation in the exon 1 promoter region of the MyF-5 gene in Chinese Bos taurus

J. A. Ujan, Zan, L. S., Ujan, S. A., Adoligbe, C., and Wang, H. B., “Back fat thickness and meat tenderness are associated with a 526 T→A mutation in the exon 1 promoter region of the MyF-5 gene in Chinese Bos taurus”, vol. 10, pp. 3070-3079, 2011.

Qualitative trait loci (QTL) for growth and meat quality traits in cattle (Bos taurus) have been previously mapped to three chromosome regions, 0 to 30, 55 to 70, and 70 to 80 cM on chromosome 5. We evaluated the allele frequencies and gene-specific single nucleotide polymorphisms (SNPs) of bovine myogenic factor 5 (MyF-5) in the QTL regions and their associations with live weight and meat characteristics in indigenous Chinese cattle breeds. PCR-SSCP methodology showed a T>A mutation at 526 bp.

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