Apoptosis

Fas/FasL in the immune pathogenesis of severe aplastic anemia

C. Y. Liu, Fu, R., Wang, H. Q., Li, L. J., Liu, H., Guan, J., Wang, T., Qi, W. W., Ruan, E. B., Qu, W., Wang, G. J., Liu, H., Wu, Y. H., Song, J., Xing, L. M., and Shao, Z. H., Fas/FasL in the immune pathogenesis of severe aplastic anemia, vol. 13, pp. 4083-4088, 2014.

Fas/FasL protein expression of bone marrow hematopoietic cells was investigated in severe aplastic anemia (SAA) patients. Fas expression was evaluated in CD34+, GlycoA+, CD33+, and CD14+ cells labeled with monoclonal antibodies in newly diagnosed and remission SAA patients along with normal controls. FasL expression was evaluated in CD8+ cells in the same manner.

Roles of Fas/Fasl, Bcl-2/Bax, and Caspase-8 in rat nonalcoholic fatty liver disease pathogenesis

C. P. Li, Li, J. H., He, S. Y., Li, P., and Zhong, X. L., Roles of Fas/Fasl, Bcl-2/Bax, and Caspase-8 in rat nonalcoholic fatty liver disease pathogenesis, vol. 13, pp. 3991-3999, 2014.

The aim of this study was to investigate the roles of Fas/FasL, Bcl-2/Bax, and Caspase-8 mRNA expressions in nonalcoholic fatty liver disease (NAFLD). The apoptosis percentage was measured by flow cytometry, the immunohistochemical assay was performed for the determination of Fas, FasL, Bcl-2, and Bax expressions, and a real-time polymerase chain reaction (PCR) assay was performed to detect Caspase-8 mRNA expression.

In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells

M. A. Ali, M. Farah, A., Al-Hemaid, F. M., and Abou-Tarboush, F. M., In vitro cytotoxicity screening of wild plant extracts from Saudi Arabia on human breast adenocarcinoma cells, vol. 13, pp. 3981-3990, 2014.

This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the extract of Lavandula dentata (Lamiaceae) was identified, and we analyzed its anticancer potential by testing antiproliferative and apoptotic activity. Our results clearly show that ethanolic extract of L.

Wnt/β-catenin aids in regulating the proliferation of hepG2 cells mediated by thy-1

B. Q. Cheng, Jiang, Y., Zhu, Q., and Lin, W. G., Wnt/β-catenin aids in regulating the proliferation of hepG2 cells mediated by thy-1, vol. 13, pp. 5115-5127, 2014.

Cancer stem cells have been found to play important roles in carcinoma. Although thy-1 has been identified as a potential stem cell marker of liver cancer, whether the Wnt/β-catenin signaling pathway plays an important role in regulating hepatocarcinoma proliferation and apoptosis mediated by thy-1 remains unknown. Our results showed that high thy-1 expression caused hepG2 cells transfected with a pReceiver-M29/thy-1 eukaryotic expression vector to exhibit obvious heteromorphism, featuring double or multiple nuclei and weaker apoptosis.

Molecular cloning and expression analysis of the GNAS gene in pig and porcine fibroblast cells

J. Peng, Li, Y. L., Shen, Y. F., Wang, Y., and Xu, N. Y., Molecular cloning and expression analysis of the GNAS gene in pig and porcine fibroblast cells, vol. 13, pp. 5463-5473, 2014.

The Alpha subunit of the stimulatory guanine nucleotide-binding protein (GNAS) is a complex imprinted gene. The major product of the GNAS gene is the α-subunit of the guanine nucleotide-binding protein (Gas), which plays a key role in multiple signal transduction pathways. Gas is required for the production of the receptor-stimulated intracellular cyclic adenosine monophosphate (cAMP). It has been demonstrated that an increase in the concentration of the intracellular second messenger cAMP promotes apoptosis in different tumor entities.

Effects of acrylonitrile on the pathological morphology and apoptosis of neurons in rats

C. Z. Li, Tse, L. A., Zhou, Y. L., Fan, W., Li, M., Yu, I. T. S., and Jin, T. Y., Effects of acrylonitrile on the pathological morphology and apoptosis of neurons in rats, vol. 13, pp. 5795-5802, 2014.

This study aimed to evaluate the effects of acrylonitrile (ACN) on neuronal morphology and apoptosis in rats. An ACN solution was administered to Wistar rats by gavage at doses of 0, 5, 10, or 20 mg/kg, 5 days a week for 13 weeks. The morphology of neurons and the presence of apoptosis was examined by light and electron microscope, DNA electrophoresis, immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Changes in the expression of FoxO1 and death ligand genes during follicular atresia in porcine ovary

F. Lin, Fu, Y. H., Han, J., Shen, M., Du, C. W., Li, R., Ma, X. S., and Liu, H. L., Changes in the expression of FoxO1 and death ligand genes during follicular atresia in porcine ovary, vol. 13, pp. 6638-6645, 2014.

Follicular atresia, a key phenomenon in follicle development, eliminates most of the follicles in mammalian ovaries. To investigate the molecular mechanism of follicular atresia in porcine ovaries, we investigated the mRNA expression of three important cell death ligand-receptor systems and Fox O1 in follicles with a diameter of 3-5 mm. The phosphorylation and subcellular localization of Fox O1 during granulosa cell apoptosis was also determined. TRAIL and Fas L played an important role in follicular atresia at this stage.

Protective effect of icariin on kidney in 5/6 nephrectomized rats and its mechanism

S. R. Liang, Bi, J. W., Guo, Z. L., Bai, Y., and Hu, Z., Protective effect of icariin on kidney in 5/6 nephrectomized rats and its mechanism, vol. 13, pp. 6466-6471, 2014.

The aim of this study was to investigate the renal protective effect of icariin in 5/6 nephrectomized rats and the molecular mechanisms involved. Forty male Sprague-Dawley rats were randomly divided into 5 groups: sham-operated group, 5/6 nephrectomy model group, icariin groups (20 and 40 mg/kg), and benazepril group. After 12-weeks treatment, 24-h urine and serum were collected, and urine protein, serum creatinine, and blood urea nitrogen were determined. The rats were then sacrificed and fresh kidney tissues were prepared to obtain single cell suspensions.

Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells

Y. H. Li, Chen, M., Zhang, M., Zhang, X. Q., Zhang, S., Yu, C. G., Xu, Z. M., and Zou, X. P., Inhibitory effect of survivin-targeting small interfering RNA on gastric cancer cells, vol. 13, pp. 6786-6803, 2014.

A pair of inverted repeated sequences of the gene survivin was designed for stable double-stranded RNA establishment. After stable transfection, the biological behaviors of gastric cancer cells were observed. The interference rates of survivin-targeting siRNA (siRNA-survivin) in BGC823, MKN45, SGC7901, and cisplatin-resistant SGC7901 groups were 55.363 ± 3.974, 71.433 ± 3.774, 69.433 ± 7.336, and 76.767 ± 3.541%, respectively, compared with those in the control group.

In vivo mechanism study of NGAL in rat renal ischemia-reperfusion injury

X. J. Zang, An, S. X., Feng, Z., Xia, Y. P., Song, Y., and Yu, Q., In vivo mechanism study of NGAL in rat renal ischemia-reperfusion injury, vol. 13, pp. 8740-8748, 2014.

This study aimed to determine the protective effect and mechanism of neutrophil gelatinase-associated lipocalin (NGAL) in rat kidney on ischemia/reperfusion injury (I/R). The rat I/R model was set up by cutting one kidney and clamping the contralateral renal pedicle for 45 min. Male SD rats were randomly divided into sham-operation, I/R and NGAL groups.

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